摘要
在由 2 10个测交组合组成的协青早A (协青早B 密阳 4 6 )F6 群体中 ,构建了由 12 9个RFLP、SSLP组成的连锁遗传图谱。应用QTL分析方法 ,对水稻矮败型胞质雄性不育育性恢复基因进行了定位。检测到一个主效基因和 3个效应较小的QTL(qRf 1、qRf 10 1、qRf 5 ) 。
A test cross population was established for mapping genes conditioning fertility restoration for dwarf wild abortive cytoplasmic male sterility(CMS DA) in rice. A recombinant inbred line(RIL) population consisting of 210 lines was derived from the cross Xieqingzao B/Miyang 46,in which Xieqingzao B is the maintainer line of CMS DA Xieqingzao A,and Miyang 46 is the restorer line. Each of the RILs was crossed to Xieqingzao A,and the resulting F 1s were used for phenotyping of the spikelet fertility in 1999 and 2000. A linkage map consisting of 129 RFLP and SSLP markers was constructed. QTLs having significant main effects and/or epistatic effects for spikelet fertility were determined with QTLMAPPER 1.0 of mixed linear model. Background genetic variation due to main and epistatic effects of important markers was controlled. Four QTLs having significant main effects for fertility restoration were detected. A major gene qRf 10 2 was located in interval RM258 RZ811 on the long arm of chromosome 10,and QTL qRf 1 displaying a moderate main effect was located in a position close to RG532 on chromosome 1. Two other QTLs were located on chromosome 5 ( qRf 5 ) and the short arm of chromosome 10 ( qRf 10 1 ),in which the main effect of qRf 5 bacame significant only in the absence of qRf 10 2. A significant digenic interaction was detected,which occurred between QTLs qRf 1 and qRf 5 . No significant QTL by environmental interactions and epistasis by environmental interactions were detected. Analyses on the gene effects based on markers closest to the Rf genes were made,and multi locus interactions were implied. The present results were also compared to a previous study on mapping fertility restoration genes in Milyang 46 for wild abortive CMS Zhenshan 97A,and to other published reports. It was indicated that differences on the genetic control of fertility restoration among different rice populations were mainly attributed to variations on genes with minor main effects and epistatic effects.
基金
国家农业科技跨越计划 (1999-0 1-15 )
国家 863计划 (2 0 0 1AA2 110 81)
农业部水稻生物学重点实验室开放项目~~
关键词
细胞质雄性不育
育性恢复
基因定位
水稻
cytoplasmic male sterility
fertility restoration
gene mapping
rice