泥鳅胚胎玻璃化液超低温冷冻保存研究

Cryopreservation of loach,Misgurnus anguillicaudatus,embryos by vitrification

泥鳅胚胎 (胚孔封闭期 )用 10 #玻璃化液作为低温保护剂 ,经低温 (- 196℃ )冷冻保存 17h后 ,38℃水浴解冻 ,解冻后的胚胎获得了成活。在稀释液B1、B2、B3中分别有 2 6 .7% ,15 .0 % ,3.0 %成活的胚胎。解冻后的胚孔封闭期胚胎经过 5 0h的培养 ,胚胎从胚孔封闭期发育至尾鳍出现期。

Use of a vitrified solution (composition 10) as a cryoprotectant for the cryopreservation of loach embryos is studied in this paper. Using this vitrified solution, embryos were preserved at a temperature of -196 ℃ for 17 hours. Afterward, the preserved embryos were thawed using thawing solution in warm water (38 ℃) , and revival embryos were successfully obtained. During thawing, we compared 3 kinds of thawing solution compositions (B1, B2 and B3), and the survival rates of the preserved embryos after thawing in B1, B2 and B3 thawing solutions were 26.7% , 15.0% and 3.0% respectively. After thawing, it took 50 hours for the development of revival embryos from the interval of blastopore closure to the interval of caudal fin appearance.