“红肉病”文蛤中发现的一种球形病毒的形态发生与细胞病理学

Cytopathology and morphogenesis of a spherical virus found in the Meretrix meretrix suffering from "red muscle disease" (RMD)

在患“红肉病”文蛤的消化盲囊上皮细胞中发现了一种球形病毒及其发生基质。病毒粒子呈球形 ,无囊膜 ,直径约 5 0～ 80nm ,在细胞质内形成多泡状的包涵体并进行病毒粒子的装配。受感染的细胞具有明显的病理变化 ,主要表现为核固缩或肿胀并出现核空泡 ,内质网膨胀、大部分转化泡状 ,线粒体溶解或固缩 ,溶酶体数量增多等。

From June to July 2000, specimens of Meretrix meretrix Linnaeus suffering from the 'red muscle disease'(RMD) were collected from Qinhuangdao District of Hebei Province, China. Abundant virus particles and their virogenic stroma were observed in the cytoplasm of the epithelium of digestive diverticula. The spherical-shaped virions with the size of 50～80nm in diameter lack capsula. The inclusion bodies forming in the cytoplasm are multivesicular which assemble virus particles. In the infected cells with virus, the cellular organelles presented obvious pathological changes, e. g. the endoplasmic reticulum swelling and mostly turning into vesicles, the mitochondria dissolving or condensing, the nuclear membrane expanding, dissolving and forming nuclear vesicles, and the number of lysosome increasing.