源于L1的小麦抗黄矮病基因的特异PCR标记及辅助育种的研究

PCR Markers of the BYDV Resistance Gene in Wheat Derived from Wheat-Thinopyrum intermedium Addition Line L1 and Their Application for Assisted Selection in Wheat Breeding

以小麦 -中间偃麦草附加系 L1为抗源 ,选育出抗黄矮病小麦异源易位系 HW6 4 2 ,YW4 4 3,YW2 4 3,Y9910 2 9等。本文以上述易位系及其抗源、小麦亲本作供试材料 ,鉴定出一个微卫星 (Simple sequence repeat,SSR)标记gwm37- 450 ,可跟踪、检测源于 L1的抗黄矮病基因。将难以分辨、稳定性差的 gwm37- 450 特异带分离、克隆、测序 ,根据测序结果重新设计 1对特异 PCR引物 ,转化为扩增产物有无的 SCAR(sequence characterizied amplified region)标记SC- W374 50 。利用 HW6 4 2 /中 86 0 1的 F2 群体 ,对 gwm 37- 450 和 SC- W374 50 进行分析 ,结果表明 ,二者均与抗黄矮病基因紧密连锁 ,后者较前者稳定。利用 SC- W374 50 跟踪抗黄矮病基因 ,将抗黄矮病基因、抗白粉病基因向优良小麦品种中麦 16、宛 710 7转育 ,快速选育出兼抗黄矮病、白粉病的回交植株 2 7个。实践了分子标记辅助抗黄矮病小麦育种的技术路线。

Wheat Thinopyrum intermedium translocation lines, including HW642, YW443, YW243 and Y991029 with BYDV resistance, were developed by using the addition line L1 as the resistance parent. The above translocation lines and their parents were used as test materials to screen PCR markers. A SSR marker gwm37 -450 and a SCAR marker SC W37 450 converted from gwm37 -450 were developed for the BYDV resistance. After the linkage analysis between these PCR markers and the resistance gene among F 2 population plants of HW642/Zhong8601, the results indicated that gwm37 -450 and SC W37 450 were closely linked to the BYDV resistance gene and the SCAR marker SC W37 450 was more reliable and easily scored. the three generations′ plants of the crosses among HW642 and synthetic wheat M53, wheat varieties Zhongmai 16 and Wan7107 The SCAR marker SC W37 450 were used to identifi The results indicated that the marker could be used in assisted selection. Twenty seven individual plants with resistance to both BYDV and powdery mildew were selected from the backcrossing populations.