转几丁质酶基因水稻根系微生物群落分析

Research on root microorganism community of "RCH" transgenic rice.

采用“直接观察法”和“分离培养法”对转基因水稻根系微生物群落的初步分析结果表明,导入外源水稻几丁质酶基因(RC24)的转基因水稻根内和根表的微生物群落发生显著变化,转基因水稻根部内生真菌总数显著减少,内生细菌总数显著增加,其内生细菌总数是未转基因亲本对照的10倍左右。采用“直接观察法”测得2个转基因水稻品种有内生真菌的根段率为55.2％和81.1％,而对照为100％,转基因水稻根系真菌和细菌种类与对照存在显著差异,有VA菌根泡囊的根段率显著降低。

The preliminary studies were conducted to analyze the root microorganism ecology of two transgenic rice varieties (GMR), which were genetically modified by the 'rice chitinase gene(RC24)'. The results showed that the microorganism communities of the root and root surface in two GMR varieties were different from the non-transgenic variety. They had less numbers of endo-fungi, but their numbers of endo-bacteria were about 10 times more than the control. The root-ratios with endo-fungus in two GMR varieties were 55.2% and 81. 1% , while the control was 100% . Their species of bacteria and fungi in the root systems were also different from the control. The root-ratios with VA mycorrhizic sac in GMR were low.