四川省石渠县鼠疫菌脉冲场电泳分析

Pulse-field gel electrophoresis of Yersinia pestis that isolated from Shiqu county, Sichuan province, China.

目的 确定四川省石渠县分离的鼠疫菌株的类型。方法 脉冲场电泳。结果 四川分离的鼠疫菌中出现 3个图型。从鼠疫病人分离到的菌株与青海田鼠体分离的菌株明显不同 ,而与强毒的青藏高原型菌株相似 ;青海田鼠体分离的菌株与内蒙古布氏田鼠型菌株相似 ,与其他地区的菌株均不相同。结论 青海田鼠间可能存在一种独立的鼠疫自然疫源地 ,1999年四川省石渠县发生的人间鼠疫并非由该地占优势的青海田鼠型菌株引起 ,可能在该疫源地之外感染。

Objective To verify the type of Y.pestis strains that isolated from Shiqu county, Sichuan province, China.Methods Pulse field gel electrophoresis.Results Results showed the pattern could be divided into 3 types. The strain isolated from human case is different from those strains isolated from Microtus fuscus, and similar to the Qingzang plateau strain with strong virulence. The pattern of the strains isolated from Microtus fuscus is similar to those isolated from Microtus brandti in Inner Mongolia, and different from other strains.Conclusions It was suggested that a new type of plague natural foci existed in the population of Microtus fuscus and the human plague outbreak in Shiqu county, Sichuan province in 1999 is not caused by these natural foci, most possibly infected outside.