污水养殖水的致突变性研究

Mutagenicity of Fish-Raising Water Containing Effluent

目的 探讨污水养殖水的诱变效应。方法 采用蚕豆根尖细胞微核试验及鱼外周血红细胞微核试验对排污河污水及经氧化塘预沉淀后用于生物养殖的污养水和污水养殖鱼迸行研究,以水库水样及鱼样为对照。结果 蚕豆根尖细胞微核试验表明,排污河污水、污养水的微核率明显高于对照组（P<0.01）。而清养水样与阴性对照组（蒸馏水）间差异无显著性（P>0.05）。污养鱼外周血红细胞微核率显著高于清养鱼（P<0.01）。结论 排污河污水、污养水中含有致突变物质,可通过食物链对人体产生潜在的危害。

Objective To study the mutagenicity of fish-raising water containing effluent. Methods The niuta-genieities of water samples from waste water canal, the fish-raising water(fish-pond water) treated by presedimenta-tion in oxidation tank were detected using micronucleus test of vicia faba root tip cells. The micronucleus test of peripheral red blood cells was carried out among fish raised in water containing effluent. The water samples and fish collected from reservoir as controls. Results The micronucleus test of vicia faba root tip cells showed that the micronucleus rates in treated fish-pond water and waste water were significantly higher than those of negative control group (distilled water) (P< 0.01). While the micronucleus rates showed no significant differences between reservoir water and negative control groups (P> 0.05) . The frequencies of micronucleus in peripheral red blood cells of fish raised in treated fish-pond water were significantly higher than those of fish raised in reservior water (P<0.01) . Conclusions The mutagenic substances existed in waste water and treated fish-pond water, and might have some potential hazardous effects on human health through food chain.