香豆素和寡糖素对马铃薯试管结薯的影响

EFFECT OF COUMARIN AND OLIGOSACCHARINS ON IN VITRO TUBERIZATION OF POTATO

本研究将生理活性物质香豆素、人参寡糖素和黑节草寡糖素分别加入含有BAP5.0mg/l、蔗糖8％的MS基本培养基中,用于诱导马铃薯试管结薯,并与加入矮壮素诱导的结薯效果作了比较。结果表明,在加入100mg/l香豆素时,试管结薯的数量与使用500mg/l矮壮素效果相近似。使用50mg/l人参寡糖素或10mg/l黑节草寡糖素时,试管结薯的数量明显超过或略超过用500mg/l矮壮素的效果。对4种处理下获得的试管薯进行了过氧化物酶的同工酶及酶活性的测定。结果表明,这4种不同的外源生长调节剂在使用浓度恰当时,所结薯块的过氧化物酶同工酶谱和过氧化物酶活性是近似的。

The physiological active substances coumarin and oligosaccharins extracted from Dendrobium candidum and Panax ginseng were added into the medium (MS+5.0 mg/ 1 BAP+8% sucrose) to induce in vitro tuberization of potato. The effect were compared with those of adding chlorocholine chloride (CCC) on inducing in vitro tuberization. The results showed that when 100 mg / 1 coumarin was added, the effect on amount of tuberization approximated to the effect of using 500 mg/ I CCC. When 50 mg/ 1 oligosacchrins extracted from Dendrobium candidum and 10 mg / 1 oligosaccharins extracted from Panax ginseng were used, the amount of tuberization was obviously or slightly more than that using 500 mg / 1 CCC. Analysis of peroxidase isozymes and its activity indicated that the peroxidase activity in the induced tubers and its isozymes spectrum were obviously similar when these four different growth regulators were used appropriately.