摘要
研究结果表明,墨西哥食用仙人掌嫩茎外植体在室温25℃±2℃,光照强度1500～2 000lx、光照时间12h条件下,茎萌发最适培养基为MS+6-BA 1.5mg/L+NAA 1.0mg/L,茎增殖最适培养基为MS+6-BA 1.5mg/L+NAA 0.5mg/L,茎生根最适培养基为1/2MS+NAA1.0mg/L。外植体茎萌发初始阶段先经暗培养约7d后转入光照培养有利于茎萌发和生长,茎萌发时间提早,可提高茎萌发率且茎生长健壮。
Tissue culture of Mexico edible cacti stem was studied. The results showed that the optimum culture medium for explants of cacti stem bud was MS+1.5 mg/L 6-BA+1.0mg/L NAA when room temperature was at 25℃±2℃, illumination intension 1 500~2 000 lx and illumination duration 12 h. The optimum medium was MS+5mg/L 6-BA1 + 0.5 mg/L NAA for stem bourgeon and 1/2MS +1.0 mg/L NAA for stem rooting. Culture of the explants in the dark for about 7 days and then under illumination at the early stage was good for bud shooting and growth, resulting in early bud shooting, high shooting rate and strong and healthy growth of the stem.
出处
《热带作物学报》
CSCD
2002年第2期58-61,共4页
Chinese Journal of Tropical Crops
