高三尖杉酯碱通过激活Caspase-3诱导鼻咽癌细胞凋亡

Homoharringtonine-induced apoptosis of nasopharyngeal carcinoma cells via activation of Caspase-3

目的 了解高三尖杉酯碱 (HHT)是否可通过激活Caspase 3诱导鼻咽癌细胞CNE 2Z凋亡。方法 将细胞分为 4组 :对照组、1mg/LHHT处理组 (HHT组 ) ,以及分别用Caspase抑制剂 (z VAD fmk)和Caspase 3抑制剂 (DEVD fmk)预处理 2h后 ,再用 1mg/LHHT处理的z VAD fmk +HHT组和DEVD fmk +HHT组。采用流式细胞术及荧光染色法 ,检测 4组细胞的凋亡率 ,以比色法检测它们中Caspase 3的相对活性。结果 流式细胞术和荧光染色均发现 ,HHT组的凋亡率明显高于其它各组 (P <0 .0 1) ;Caspase 3的活性于 2h开始升高 ,8h达高峰 ,明显高于其它各组 (P <0 .0 1) ,2 4h时同其它各组的差异无显著性 (P >0 .0 5 )。结论 HHT可通过激活Cas pase 3诱导CNE 2Z细胞凋亡 ,Caspase

Aim To study whether homoharringtonine(HHT) induces apoptosis of nasopharyngeal carcinoma cells CNE 2Z via the activation of Caspase 3. Methods The cells were divided into four groups (control group, HHT group, z VAD fmk+HHT group and DEVD fmk+HHT group). The apoptotic rates of the four groups were detected by flow cytometry and fluorescence staining. Caspase 3 relative activity was analyzed by colorimetric assay. Results The apoptotic rate of HHT group (the cells were treated only with 1 mg/L HHT)was much higher than that of other groups. Caspase 3 activity of HHT group began to rise at 2 h of HHT treatment and reached the peak at 8 h, which was significantly higher than that of other groups ( P <0.01). The difference of Caspase 3 activity between HHT group and other three groups had no statistical significance at 24 h ( P >0.05). Conclusion HHT induces apoptosis of CNE 2Z cells via activation of Caspase 3 and the increased Caspase 3 activity is time dependent.