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内皮素-1对大鼠睾丸间质细胞睾酮分泌的可能作用方式 被引量：1

The Effect of Endothelin-1 on Testosterone Secretion of Rat Leydig Cells In Vitro:A Possible Action Mode
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摘要 目的 :了解内皮素 - 1影响离体大鼠睾丸间质细胞睾酮分泌的可能作用方式。方法 :采用大鼠睾丸间质细胞体外孵育技术以及12 5I-睾酮放射免疫法 ,观察了缩血管肽内皮素 - 1 (ET- 1 )对间质细胞睾酮的分泌。结果 :ET- A、ET- B受体拮抗剂均可逆转 ET- 1对离体大鼠间质细胞睾酮分泌的抑制作用 ,但 T-型 Ca+ +通道阻滞剂异搏定 (VER)未表现此作用。结论 :ET- 1通过与 ET- A受体及 ET-B受体结合而发挥作用。其 Ca+ +的动员可能不是通过 T-型 Ca+ +通道的开放。 Objective: To explore the mechanism endothelin-1 affect the testosterone secretion of rat Leydig cells. Methods: After rat Leydig cells were incubated, testosterone was determined by radioimmunoassay. Results: ET-A or ET-B receptor antagonist could reverse the inhibitory effect of ET-1 on testosterone secretion, but Verapamile, a calcium-channel blocker, could not inhibit. Conclusion: ET-1 inhibits testosterone secretion of rat Leydig cell by combining to ET-A or ET-B receptor. Mobilization of Ca ++ may not via opening T- calcium-channel.
作者 周琴 徐斯凡 王晶磊 郑月慧
机构地区 华西医科大学卫生统计学教研室 江西医学院生殖内分泌研究室
出处 《生殖与避孕》 CAS CSCD 北大核心 2002年第3期140-143,共4页 Reproduction and Contraception
基金 国家科学基金资助项目 项目号 :3946 0 0 27
关键词 内皮素-1(ET-1) 内皮素受体拮抗剂 异搏定 大鼠 endothelin-1, endothelin receptor antagonist,Verapamile,rat
分类号 Q492.4 [生物学—生理学]
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参考文献11

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同被引文献19

  • 1刘建中,郭海彬,邓春华,欧咏虹,彭爱萍.大鼠睾丸Leydig细胞的培养和鉴定[J].中华男科学杂志,2006,12(1):14-17. 被引量：40
  • 2薛庆善.体外培养的原理和技术[M].北京:科学出版社,2001.54-63.
  • 3ZHANG Y, GE R, HARDY M P. Androgen-forming stem Leydig cells : identification, function and therapeutic potential[ J ]. Dis Markers, 2008, 24 (4/5) : 277 - 286.
  • 4KLINEFELTER G, LASKEY J, AMANN R. Statin drugs markedly inhibit testosterone production by rat Leydig cells in vitro: implications for men [ J ]. Reprod Toxicol, 2014, 45C: 52-58.
  • 5DEMIRBAS D, WYMAN A R, SHIMIZU-ALBERGINE M, et al. A yeast-based chemical screen identifies a PDE inhibitor that elevates steroidogenesis in mouse Leydig cells via PDE8 and PDE4 inhibition [ J ]. PloS one, 2013, 8(8): e71279.
  • 6ZHANG L, WANG H, YANG Y, et al. NGF induces a- dult stem Leydig cells to proliferate and differentiate dur- ing Leydig cell regeneration ~ J ]. Biochem Biophys Res Commun, 2013, 436(2): 300-305.
  • 7SUN J, ZHONG L, ZHU Y, et al. Research on the isola- tion of mouse Leydig cells using differential digestion with a low concentration of collagenase [ J 1- J Reprod Dev,2011, 57(3): 433-436.
  • 8SHARMA R, PAL P, RAJALAKSHMI M. Isolation and culture of Leydig cells from adult rats I J 1. Indian J Clin Biochem, 2006, 21(1): 27-33.
  • 9BILINSKA B, KOTULA-BALAK M, SADOWSKA J. Morphology and function of human Leydig cells in vitro. Immunocytochemical and radioimmunological analyses [J]. EurJHistochem, 2009, 53(1): 35-42.
  • 10LEJEUNE H, SANCHEZ P, SAEZ J. Enhancement of long-term testosterone secretion and steroidogenic enzymeexpression in human Leydig ceils by co-culture with hu- man Sertoli cell-enriched preparations [ J 1. Int J Androl, 1998, 21(3) : 129 -140.

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生殖与避孕
2002年 第3期

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