多沙唑嗪抑制人血管平滑肌细胞增殖的实验研究

Effect of Doxazosin on proliferation of human aortic vascular smooth muscle cells

目的探讨多沙唑嗪对人血管平滑肌细胞(VSMCS)增殖的抑制作用。方法将0.1、1、10、25μmol/L的多沙唑嗪(Doxazosin,Dox)作用于体外培养的VSMCS,采用氚-胸腺嘧啶核苷(3H-TdR)掺入的方法检测细胞的增殖。结果在1、10、25μmol/L的Dox作用下,VSMCS的3H-TdR的掺入量分别为(983±58.7)cpm、(730±54.9)cpm、(500±64.1)cpm,显著低于对照组的(1270±96.5)cpm(P<0.01),并表现为剂量依赖性(P<0.01)。结论多沙唑嗪能够抑制人VSMCS的增殖,对经皮腔内冠状动脉成形术(PTCA)后再狭窄可能有防治作用。

Objective To investigate the effects of Doxazosin on the proliferation of human aortic vascular smooth muscle cells(VSMC S )cultivated in vitro.Methods Various concentrations of Doxazosin(0.1,1,10,25μmol/L)were added to the cultivated VSMC S .The cell proliferation was detected by 3 H-TdR incorporation.Results 3 H-TdR incorporation in various concentrations of Doxazosin(1,10,25μmol/L)were(1037.6±119.4),(728.4±104.4),(462.1±112.3)cpm respectively,there were significant difference compared with control group (1270±96.5)cpm,P<0.01) each other.Doxazosin had antiproliferative effect on VSMC in the dose-dependent manners.Conclusion These results suggest that Doxazosin inhibits the proliferation of human VSMC S and is useful in preventing restenosis after PTCA.