三种检测泌尿生殖道沙眼衣原体方法的比较

Evaluation of Three Laboratory Diagnosis of Chlamydia Trachomatis Infection of Urogenital Tract

目的 比较三种泌尿生殖道沙眼衣原体实验室检测方法。 方法 用单克隆抗体免疫荧光法 (IFA)、细胞培养法 (CC)和聚合酶链法 (PCR)平行检测 10 7例非淋泌尿生殖道炎症患者男性尿道或女性宫颈上皮细胞 ,比较三种方法的阳性检出率、敏感性、特异性和试验效率等。 结果 10 7例标本经 IFA、CC、PCR检测 ,分别有 16 .8%、19.6 %、2 0 .6 %阳性 ;以细胞培养法结果为标准 ,IFA和 PCR的敏感性分别为 81.0 %和 10 0 % ;特异性均为 98.8% ;试验效率分别为 95 .3%和 99.1%。三种方法平行检测相同标本结果无差异。 结论 这三种方法均可应用于沙眼衣原体的诊断。

Objective Aim To evaluate three laboratory diagnosis of Chlamydia trachomatis infection of urogenital tract. Methods Specimens were collected from 107 cases visiting the outpatient clinic for Sexually Transmitted Diseases. The specimens were assayed by using immunology fluorescent antibody technology(IFA), cell culture(CC) and polymerase chain reaction(PCR). Result Positive rates by the three methods were 16.8%,19.6% and 20.6%, respectively. No significant statistical difference was found among these positive rates(P>0.05). Compared with cell culture as the gold standard, the sensitivity of IFA and PCR were 81.0% and 100%, respectively; the specificity was 98.8% ; and the detection efficiency of them were 95.3% and 99.1%, respectively . Conclusion The three methods can all be used to diagnosis of Chlamydia trachomatis infection.