前列腺素E_2对重症急性胰腺炎大鼠血清IL-10和IL-1β的影响

Effect of immuno-intervention with prosglandin E_2 on interleukin-10 and interleukin-1β in rats with acute severe pancreatitis

目的 :探讨前列腺素E2 (PGE2 )对重症急性胰腺炎大鼠血清IL 1β和IL 10调节作用。 方法 :SD大鼠 91只随机分为 4组 :假手术组 (n =7) ,ANP组 ,PGE2 前、后干预组 (后三组每组 2 8只 ,分别随机分为 1h、3h、6h、12h时段组 ,n =7)。大鼠ANP模型用 5 %牛磺胆酸钠制备。前干预组在ANP诱导前 2h肌肉注射 15 0μg·kg-1PGE2 ,后干预组在ANP模型完成时即刻肌肉注射 15 0 μg·kg-1PGE2 。采用ELISA检测各组不同时段大鼠血清IL 10与IL 1β含量 ,观察PGE2 前、后干预对大鼠血清IL 10与IL 1β的调节作用。 结果 :PGE2 前、后干预组大鼠血清IL 10峰值分别位于 3h和 6h时段 ,均显著高于ASP组 (P <0 .0 0 1) ;前、后干预组所有时段大鼠血清IL 1β均比ASP组降低 (P <0 .0 0 1) ,差异有显著性 ;前干预组 3h时段IL 10峰值 (2 31pg/ml)比后干预组IL 10相应时段 (2 0 9pg/ml)高 (P <0 .0 1) ,差异有显著性 ;6h时段IL 10 (5 4.0 7pg/ml )比后干预组 (118.2 6pg/ml)低 ,差异有显著性 (P <0 .0 0 1)。前干预组 1～ 6h时段大鼠血清IL 1β均显著低于后干预组 (P <0 .0 5 ,P <0 .0 0 1)。结论 :PGE2 能上调大鼠血清IL 10 ,并降低IL 1β含量。

Objective:To explore the effect of immunointervention with prostaglandin E 2(PGE 2 ) on interleukin-10(IL-10) and interleukin-1β (IL-1β) in rats with acute necrotizing pancreatitis(ANP).Methods:91 SD rats were randomly divided into 4 groups:sham operation group (n=7), ANP group, pre-intervention group and post-intervention group(n=28, respectively), which were randomly sub-divided into 4 different time groups (1h, 3h, 6h, 12h, n=7, respectively). ANP model of rats was induced by retrograde injection of 5% sodium taurocholate in the pancreatic duct. Pre-intervention group intramuscularly injected into 150 μg·kg -1 PGE 2 before 2 hours induction of ASP and the same dose of post-intervention group of PGE 2 administration were the same above dose after ASP model immediately. The serum levels of IL-10 and IL-1β in rats were measured by specific enzyme linked immuno-sorbent assay (ELISA) at different time (1h, 3h, 6h, 12h), respectively.Results: The serum IL-10 levels enhances significantly either the pre-intervention (3h) with PGE 2 group or post-intervention (6h) with PGE 2 group compared with the control group (ASP) (P<0.001,respectitively).Serum IL-1β levels of both groups treated with PGE 2 compared with control group (ASP) of different times were significantly reducted (P<0.001, respectively).Conclusion: The present study suggests that either pre-intervention or post-intervention with PGE 2 enhance IL-10 levels,and regulate the IL-1β levels in rats to a lower level.[FK(WB80011?6]