耐药相关基因在胃癌组织中的表达及其临床意义

Expression of multidrug resistance in gastric carcinoma and its clinical significance

目的 :探讨耐药相关基因P糖蛋白 (P gp)、拓扑异构酶Ⅱ (TOPOⅡ )、谷胱甘肽 S 转移酶 (GST π)在胃癌组织中的表达及其临床意义。方法 :应用免疫组化检测 90例术前未使用过化疗、放疗及免疫治疗的胃癌组织中的P gp、TOPOⅡ和GST π的表达。结果 :P gp、TOPOⅡ、GST π在胃癌组织中的表达分别为 82 .2 %(74/ 90 )、78.9% (71/ 90 )、87.8% (79/ 90 ) ;P gp和GST π的表达与胃癌的分化程度 ,浸润深度和淋巴结转移差异均无显著性 (P >0 .0 5 )。TOPOⅡ表达与胃癌的组织学类型差异有显著性 (P <0 .0 5 ) ,与胃癌的浸润深度和淋巴结转移差异均无显著性 (P >0 .0 5 )。各耐药相关基因之间的共同表达无明显的相关性 (P >0 .0 5 )。结论 :P gp和GST π的阳性表达可否作为胃癌病人的预后判断指标尚不能明确 ,TOPOⅡ阳性表达的高低可以作为胃癌预后的一个指标。各耐药相关基因的检测有利于指导化疗用药。

Objective:To investigate the expression of multidrug resistance(MDR) P-gp,TOPOⅡ,GST-π in gastric carcinoma.Methods:90 patients with gastric carcinoma,which had not been treated by chemotherapy radiotherapy or immunotherapy before surgery,were studied for expression of P-gp TOPOⅡand GST-πusing immunohistochemistry.Results:P-gp, TOPOⅡand GST-πwere detected in 82.2%(74/90),78.9%(71/90)and 87.8%(79/90), respectively. Correlation was not observed between P-gp ,GST-π expression and hisological grading, invasive depth and lymph node metastases(P>0.05). TOPOⅡ expression was not correlated with invasive depth and lymph node metastases(P>0.05),but with hisologocal grading (P<0.05). Conclusion:The expression of TOPOⅡ is an indicator for prognosis of gastric carcinoma while the expression of P-gp, TOPOⅡand GST-π can be factors in clinical chemoresistance in gastric carcinoma.[FK(WB80011?6]