乙型肝炎四项指标消长分析及其相互关系研究

Analyses and interrelations of HBV DNA,PHSA-Re ,HBcAg and HBeAg

目的探讨HBVDNA与乙型肝炎聚合人血清白蛋白受体(PHSA-Re)、核心抗原(HBcAg)、e抗原(HBeAg)的相互关系。方法采用荧光定量PCR法检测HBV-DNA,对其中浓度不同阳性102例和阴性36例进一步检测PHSA-Re,HBcAg和HBeAg。结果102例HBVDNA阳性中PHSA-Re、HBcAg、HBeAg的阳性率分别占86.27%、82.35%、72.55%。36例HBVDNA阴性中PHSA-Re、HBcAg、HBeAg的阳性率分别占38.89%、80.56%、11.11%。PHSA-Re浓度与HBVDNA含量呈正相关递增。结论HBVDNA阴性患者HBV复制和感染仍有并存,四项指标联合检测更能客观反映HBV复制与感染程度。

Objective To study the interrelations of HBV DNA,PHSA-Re ,HBcAg and HBeAg.Methods Contents of serum HBV DNA in patients with hepatitis B virus were determined by PCR-Fluorescence quanification:The serum PHSA-Re ,HBcAg and HBeAg were determined in102positive cases with different concentrations of HBV DNA and in36cases with negative HBV DNA.Results The positive rates of PHSA-Re ,HBcAg and HBeAg were86.27%,82.35%,and72.55%in the102positively cases and38.89%,80.56%and11.11%in the36cases respectively.The increased of PHSA-Re concentrations was positively correlated with HBV DNA content.Con clu sion Infection and replication of hepatitis B virus in patients with negative HBV DNA still coexist,and combined determination of the four parameters can reflect objectively the degree of replication and infection.