蚓激酶溶栓作用的实验研究

The thrombolysis effects of lumbrokinase on the arterioveneous shunt thrombosis in rabbits

目的 研究蚓激酶在兔动 静脉旁路血栓形成模型中的溶栓作用。方法 将实验动物随机分为 6组 :模型组 ,生理盐水对照组 ,蚓激酶小剂量组 (2 5 μg kg) ,中剂量组 (5 0 μg kg) ,大剂量组 (10 0 μg kg) ,尿激酶阳性对照组 (2×10 4 U kg)。采用家兔颈动 静脉旁路模型 ,测定蚓激酶的溶栓作用 ;心脏取血 ,测定蚓激酶对血浆纤维蛋白原 (FIB)、血浆优球蛋白溶解时间 (ELT)、纤维蛋白裂解产物 (FDP)等生化指标的影响。结果 蚓激酶中剂量组与大剂量组可使ELT缩短 ,使血栓重量减轻 ;蚓激酶大、中、小剂量组可使FDP显著增多 ;蚓激酶对FIB影响不明显。结论 蚓激酶有抑制血栓形成 ,激活纤溶活性的作用 ,具有较好溶栓效力。

Objective To evaluate the thrombolysis effect of lumbrokinase in rabbit model of arterioveneous shunt thrombosis.Methods The arterioveneous shunt thrombosis model was used to study the thrombolysis effect of lumbrokinase in rabbits. The rabbits were randomly devided into 6 groups:model group, control group, lumbrokinase 25μg/kg group, lumbrokinase 50μg/kg group, lumbrokinase 100μg/kg group and urokinase 2×10 4U/kg group. 15 minutes after operation, the drugs were administered introveneously in each group. 3 hours later, the ELT,FDP,FIB and the weight of thrombus were measured in each one. Results Lumbrokinase could shorten ELT, reduce the weight of thrombus and increase FDP. However lumbrokinase had no influence on FIB.Conclusion Lumbrokinase possesses the effect of preventing thrombosis and the effect of thrombolysis.