糖尿病视网膜病变患者玻璃体中肝细胞生长因子的含量检测

A comparative study of hepatocyte growth factor levels in the vitreous of patients with diabetic retinopathy

目的 定量研究肝细胞生长因子 (hepatocyte growth factor,HGF)在糖尿病视网膜病变(diabetic retinopathy,DR)患者玻璃体中的水平 ,探讨 HGF在增生性糖尿病视网膜病变 (proliferative dia-betic retinopathy,PDR)等新生血管形成病理过程中的作用。 方法 采用双夹心酶联免疫吸附测定法检测对照组 10只眼玻璃体以及单纯型 DR组 7只眼、PDR组 33只眼和其它与新生血管生成有关的视网膜疾病组 8只眼玻璃体切割手术中所取玻璃体内 HGF的含量。PDR组中无虹膜新生血管者 2 4只眼 ,伴虹膜新生血管者 9只眼。 结果 玻璃体中 HGF的含量对照组为 (3.34± 1.9)μg/ L ;单纯型 DR组为 (4.8±2 .5 ) μg/ L;PDR组中不伴虹膜新生血管生成者为 (13.0± 5 .2 ) μg/ L;PDR伴有虹膜新生血管生成者为(18.6± 7.2 ) μg/ L,其它与新生血管生成有关的视网膜疾病组为 (12 .1± 8.9) μg/ L。PDR组和其它与新生血管生成有关的视网膜疾病组玻璃体中 HGF的含量比对照组显著升高 (t=6 .4 9,5 .70 ,3.0 1,P<0 .0 1) ;PDR组中伴有虹膜新生血管生成者较 PDR不伴虹膜新生血管生成者以及单纯型 DR组玻璃体中 HGF的含量均高 ,其差异有显著性意义 (t=2 .4 7,P<0 .0 5或 t=4 .84 ,P<0 .0 1)。 结论 在

Objective To determine the concentration of hepatocyte growth factor (HGF) in vitreous of diabetic retinopathy (DR) and investigate the result and influence of HGF in neovascularization in proliferative diabetic retinopathy (PDR). Methods The high sensitive sandwich enzyme linked immunosorbent assay (ELISA) was used to measure the level of HGF in vitreous of normal group for 10 eyes, simple DR group for 7 eyes, PDR without iridal neovascularization group for 24 eyes, PDR with neovascularization of iris group in 9 eyes, other retinopathy group associated with neovascularization for 8 eyes. Results The mean value of HGF level in vitreous of the former groups was: (3.3 ±1.9) μg/L in normal group; (4.8±2.5) μg/L in simple DR group; (13.0 ±5.2) μg/L in PDR without iridal neovascularization group; (18.6±7.2) μg/L in PDR with neovascularization of iris group; (12.1±8.9) μg/L in other retinopathy associated with neovascularization group. Stastistics showed that HGF level in PDR group and other retinal diseases associated with neovascularization were significantly higher than that in normal group ( P< 0.01), PDR with neovascularization of iris group showed a higher mean vitreous HGF concentration than those in PDR without iridal neovascularization and simple DR group ( P< 0.05 or P< 0.01). Conclusion Our results indicate that vitreous HGF may play an important role in retinal neovascularization in PDR and other retinal diseases.