SD大鼠视网膜神经细胞培养上清液对胚胎干细胞体外分化的诱导作用

The preliminary study on commitment differentiation of embryonic stem cells induced by the medium of cultured retinal neurons of SD rats

目的 探讨视网膜神经细胞培养的上清液对胚胎干细胞 (embryonic stem cells,ES)体外分化的诱导作用。 方法 收集 SD大鼠视网膜神经细胞培养上清液 ,抽滤后按 2∶ 3比例与 DMEM培养液混合 ,用该混合液进行 ES细胞的诱导分化 ,每天倒置相差显微镜观察 ES细胞的生长及分化情况 ,对诱导分化后的细胞进行神经丝蛋白 (nellcofilamentprotein,NFP)免疫组织化学检查。 结果 加入了视网膜神经细胞培养上清液的 ES细胞生长出类似神经细胞突起样结构 ,NFP免疫组织化学染色阳性。 结论 SD大鼠视网膜神经细胞培养的上清液具有诱导 ES细胞向神经细胞分化的作用。

Objective To investigate the possibility of commitment differentiation of embryonic stem cells induced by the medium of cultured retinal neurons of SD rats. Methods The medium from cultured retinal neurons of SD rats were collected, sterilized and mixed with DMEM medium according to 2∶3 proportion, ES cells were cultured with these mixed medium and were observed under the phase contrast microscope daily, the induced cells were identified by NF immunohistochemistry methods. Results The ES cells cultured with these mixed medium can differentiate into neuron like structure, and the induced cells were positive in NF immunofluorescence staining. Conclusion The medium from cultured retinal neurons of SD rats can induce ES cells commitment differentiation into neuron like structure.