摘要
目的 :观察去卵巢 ( OVX)大鼠纹状体多巴胺 ( DA)神经递质的含量、释放及其受体活性的变化 ,并于体外进一步观察雌二醇对纹状体脑片释放 DA的作用特点。方法 :DA含量采用高效液相电化学检测器检测 ,其他采用同位素标记法。结果 :OVX鼠纹状体 DA含量明显低于正常组 ;纹状体脑片 DA释放显著减少 ;DA摄取未见改变 ;纹状体 DA受体活性在 OVX鼠是对照组的 2倍 ,在下丘脑无明显改变。体外观察到雌二醇对纹状体 DA释放作用的剂量 -效应曲线呈钟形。结论 :雌激素对脑内纹状体 DA功能有明显调节作用 ,并存在部位。
Objective: To study the changes of content, release and receptor binding activity of striatum dopamine (DA) in ovariectomized (OVX) Wistar rats, and further investigate the effects of estrodial-17 β on the dopamine release of striatum slices in vitro. Methods: The content of DA was determined with HPLC-ED. Its release and receptor binding activity were measured with radioactivity assay. Results: The content of DA decreased significantly in OVX rats, but there was no change in its uptaking. The receptor activity increased 2 times in OVX rats as compared with the control group.The effect of estrodial-17 β on the DA release of striatum slices in vitro showed in bell-shaped dose-response curve.Conclusion: Estrogen could regulate the function of striatum dopamine neuron, and this action was site and dose dependent.
出处
《生殖医学杂志》
CAS
2002年第3期140-144,共5页
Journal of Reproductive Medicine
基金
国家科技部重点 973项目 (J2 0 0 0 5 710 0 8)
