摘要
目的:建立人血浆中头孢羟氨苄浓度的测定方法。方法;色谱柱为Hyersil ODS柱(4.6mm×250mm,10μm),流动相:0.01 mol/L NaH_2PO_4-乙腈(96:4),流速1.0ml/min,检测波长230nm。结果:头孢羟氨苄的线性范围为0.5～40μg/ml,回归方程为y=0.0184+0.1235x(r=0.9999)。最低检测浓度为0.25μg/ml,日内RSD(％)为2.1～2.9,日间RSD(％)为4.5～7.1。结论:本法操作简便,重复性好,结果准确。
AIM: The purpose is to establish a method for the determination of cefadroxil by HPLC. METHODS: The Hypersil ODS (4. 6 mm×250 mm, 10μm)column was used, mobile phase was 0. 01 mol/L sodium dihydrogenphosphate-acetonitrile(94 : 6). The flow-rate was 1. 0 ml/min. The detection wavelength was 230 nm. RESULTS: The range of the calibration was 0. 5-40μg/ml (y = 0. 0184 + 0. 1235x,r=0. 9999). The minimal detectable plasma concentration was 0. 25 μg/ml. RSD within-day was 2. 1%-2. 9%. RSD day-to-day was 4. 5%-7. 1%. CONCLUSION: The method is simple and accurate.
出处
《江苏药学与临床研究》
2002年第2期31-33,共3页
Jiangsu Pharmacertical and Clinical Research
