前列腺癌与雄激素受体基因(CAG)n重复多态性的关系

Study on the polymorphism of (CAG)n repeats within androgen receptor gene in patients with prostate cancer

目的 探讨前列腺癌 (PC)与雄激素受体 (AR)基因 (CAG)n重复多态性的关系。 方法 应用DNA双链循环测序方法对 34例PC组织与癌旁正常组织、2例PC患者外周血白细胞内的AR基因 (CAG)n重复数进行测定。 结果 同一PC患者的癌组织与癌旁正常组织 (CAG)n重复数相同 ;36例患者癌组织AR基因 (CAG)n呈重复多态性 ,平均 2 0 .0 6 ,显著低于正常组织 ,差别有显著性意义 (P <0 .0 5 ) ;不同分化程度的癌组织 (CAG)n重复数差别无显著性意义 (P >0 .0 5 )。 结论 AR基因 (CAG)n重复数改变的体细胞突变在PC癌细胞中罕见 。

Objective To study the relationship between polymorphic (CAG)n repeats of androgen receptor(AR) gene and occurrence of prostate cancer (PC). Methods (CAG)n repeats of both malignant cells and adjacent nonmalignant cells from 34 paraffin embedded PCs, and that of peripheral blood cells from 2 PC patients were assessed using dsDNA cycle sequencing. Results The number of (CAG)n repeats in malignant cells was equal to that in adjacent nonmalignant cells from the same paraffin embedded PC; the mean of (CAG)n repeats in the 36 PC patients was 20.06 which was significantly smaller than that of normal men ( P <0.05), although no significant difference in the polymorphic distribution of (CAG)n was found among various grades of PC( P >0.05). Conclusions Shortening of (CAG)n repeats within AR gene may be associated with the occurrence of PC.