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BCG HSP65-人HER-2/neuT细胞表位融合蛋白基因的扩增及序列分析 被引量：2

Amplification and sequence analysis of fusion protein gene of BCG-HSP65 and human HER-2/neu T cell epitope
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摘要 目的 :获得卡介苗热休克蛋白 6 5 (BCGHSP6 5 )与人HER 2 /neuT细胞表位 (GP2 )构成的融合蛋白 (BCGHSP6 5 GP2 )基因 ,并对其进行测序。方法 :从卡介苗结核杆菌中提取其基因组DNA ,设计一对寡核苷酸引物 ,采用聚合酶链反应 (PCR)获得BCGHSP6 5基因 ,再设计一对寡核苷酸引物 ,再次PCR ,获得BCGHSP6 5 GP2 基因 ,DNA序列分析BCGHSP6 5 GP2 基因。结果 :本实验采用两轮PCR方法获得的基因片段长度为 170 0bp ,DNA序列分析确证为编码BCGHSP6 5 GP2 融合蛋白的序列。结论 :采用PCR获得BCGHSP6 5 GP2 基因序列是正确的 。 Objective:To obtain the sequence of fusion protein gene of BCG HSP65 and human HER 2/neu T cell epitope.Methods:BCG HSP65 GP 2 gene was obtained by using two round PCR and then sequenced.Results:The length of the reconstructed gene is 1 700 bp. Sequencing analysis confirmed that it was the right sequence encoding fusion protein of BCG HSP65 GP 2. Conclusions:The sequence of BCG HSP65 GP 2 obtained by two round PCR is corrected.It has laid the foundation for the further researching work on the cloning and expression of BCG HSP65 GP 2.
作者 王丽娟 吴士彬 于永利 王丽颖
机构地区 蚌埠医学院病原生物学教研室 安徽省蚌埠市第三人民医院泌尿外科 吉林大学基础医学院免疫学教研室 吉林大学基础医学院分子生物学教研室
出处 《蚌埠医学院学报》 CAS 2002年第4期292-294,共3页 Journal of Bengbu Medical College
关键词 人HER-2/neu T细胞表位 基因表达调节 卡介苗热休克蛋白65 基因扩增 基因序列 免疫疗法 肿瘤疫苗 肿瘤治疗 gene expression regulation BCG vaccine heat shock protein 65 DNA
分类号 R730.1 [医药卫生—肿瘤]
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参考文献5

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  • 7Yamada H,Matsumoto S,Matsumoto T,et al.Muxine IL-2 se creting recombinant bacillus cal mette-guerin augments macrophage-me ated cytotoxicity against murine bladder cancer MBT2[J].J Urol,2000,164:526-531.
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蚌埠医学院学报
2002年 第4期

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