期刊文献+

BCG HSP65-人HER-2/neuT细胞表位融合蛋白基因的扩增及序列分析 被引量:2

Amplification and sequence analysis of fusion protein gene of BCG-HSP65 and human HER-2/neu T cell epitope
下载PDF
导出
摘要 目的 :获得卡介苗热休克蛋白 6 5 (BCGHSP6 5 )与人HER 2 /neuT细胞表位 (GP2 )构成的融合蛋白 (BCGHSP6 5 GP2 )基因 ,并对其进行测序。方法 :从卡介苗结核杆菌中提取其基因组DNA ,设计一对寡核苷酸引物 ,采用聚合酶链反应 (PCR)获得BCGHSP6 5基因 ,再设计一对寡核苷酸引物 ,再次PCR ,获得BCGHSP6 5 GP2 基因 ,DNA序列分析BCGHSP6 5 GP2 基因。结果 :本实验采用两轮PCR方法获得的基因片段长度为 170 0bp ,DNA序列分析确证为编码BCGHSP6 5 GP2 融合蛋白的序列。结论 :采用PCR获得BCGHSP6 5 GP2 基因序列是正确的 。 Objective:To obtain the sequence of fusion protein gene of BCG HSP65 and human HER 2/neu T cell epitope.Methods:BCG HSP65 GP 2 gene was obtained by using two round PCR and then sequenced.Results:The length of the reconstructed gene is 1 700 bp. Sequencing analysis confirmed that it was the right sequence encoding fusion protein of BCG HSP65 GP 2. Conclusions:The sequence of BCG HSP65 GP 2 obtained by two round PCR is corrected.It has laid the foundation for the further researching work on the cloning and expression of BCG HSP65 GP 2.
出处 《蚌埠医学院学报》 CAS 2002年第4期292-294,共3页 Journal of Bengbu Medical College
  • 相关文献

参考文献5

  • 1Disis ML,Smith JW,Murphy AE,et al.In vitro generation of human cytolytic T-cells specific for peptides derived from the HER-2/neu protooncogene protein[].Cancer Research.1994
  • 2Heikema A,Agsteribbe E,Wilschut J,et al.Generation of heat shock protein-based vaccines by intracellular loading of gp96 with antigenic peptides[].Immunology Letters.1997
  • 3Peoples GE,Goedegebuure PS,Smith R,et al.Breast and ovarian cancer-specific cytotoxic T lymphocytes recognize the same HER2/neu-derived peptide[].Proceedings of the National Academy of Sciences of the United States of America.1995
  • 4Cole ST,Brosch R,Parkhill J,et al.Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence[].Nature.1998
  • 5Blachere NE,Li Z,Chandawarkar RY,et al.Heat shock proteinpeptide complexes, reconstituted in vitro, elicit peptide-specific cytotoxic T lymphocyte response and tumor immunity[].The Journal of Experimental Medicine.1997

同被引文献9

  • 1Baxi M K,Deregt D,Robertson J,et al.Recombinant bovine adenovirus type 3 expressing bovine viral diar-rhea virus glycoprotein Einduces an immune response in cotton rats[J].Virology,2000,278 (1):234-243.
  • 2Delogu G,Brennan M J.Comparative antigens of Mycobacterium tuberculosis immune response to PE and PE PGRS[J].Infect Immun,2001,69 (9):5606-5611.
  • 3Nasci mento I P,Dias W O,Mazzantini R P,et al.Re-combinant mycobacterium bovis BCG expressing per-tussis toxin subunit Slinduces protection against an in-tracerebral challenge with livebordetella pertussis in mice[J].lnfect Immun,2000,68:4877-4883.
  • 4Naoya O,Matsuoka H,Hiroko N,et al.Protective re-sponses against experimental mycobacterium lepra in-fection in mice induced by recombinant bacillus Calmette-Guerin overproducing three putative protec-tive antigen candidates[J].Vaccine,2001,19:1906-1910.
  • 5殷震,刘景华.动物病毒学[M].2版.北京:科学出版社,1990:645-652.
  • 6Grigera P R,Marzocca M P,Capozzo A V,et al.Pres-ence of bovine viral diarrhea virus (BVDV)E2 glyco-protein in VSV recombinant particles and induction of neutralizing BVDV antibodies in mice[J].Virus Res,2000,69(1):3-15.
  • 7Yamada H,Matsumoto S,Matsumoto T,et al.Muxine IL-2 se creting recombinant bacillus cal mette-guerin augments macrophage-me ated cytotoxicity against murine bladder cancer MBT2[J].J Urol,2000,164:526-531.
  • 8Horwitz M A,Harth G,Dillon B J,et al.Recombinant bacillus calmette-guerin (BCG) vaccines expressing the Mycobacterium tuberculosis 30-kDa major secretory protein induce greater protective immunity against tuberculosis than conventional BCG vaccines in a highly susceptible animal model[J].Proc Natl Acad Sci USA,2000,97 (25):13853-13858.
  • 9Dziadek J,Madiraju MVVS,Rutherford S A.Physiological consequences associated with overproduction of Mycobacterium tuberculosis[J].Microbiology,2002,148:961-971.

引证文献2

二级引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部