ZDY101和SZ201对α-分泌性淀粉样前体蛋白生成的影响

The Effect of ZDY101 and SZ201 on α-sAPP Generation

目的探讨ZDY10 1和SZ2 0 1及二者不同配比联合应用对HEK2 93sw细胞生成α -分泌性淀粉样前体蛋白的影响。 方法用Westernblot法检测HEK2 93sw细胞生成的α -sAPP含量 ,观察ZDY10 1、SZ2 0 1及二者不同配比联合应用在 2 4～ 48h、48～ 72h时段内α -sAPP生成量的变化。 结果 2 4～ 48h时段的实验中 ,ZDY10 1和SZ2 0 1浓度分别为 1× 10 - 5mol L时 ,α -sAPP含量分别为 2 .0 6± 0 .73、2 .6 4± 1.2 1,明显高于DMSO对照组的 1.0 0(P <0 .0 1) ;48～ 72h时段的实验中 ,ZDY10 1和SZ2 0 1单独应用且浓度分别为 1× 10 5mol L时 ,α -sAPP含量也明显高于DMSO对照组的 1.0 0 (P <0 .0 1)。二者相加的效果高于ZDY 10 1、SZ2 0 1单独应用 (P <0 .0 5 )。如果二者联合应用 ,降低各自的浓度 ,总浓度仍为 1× 10 - 5mol L时 ,提高α -sAPP含量的效果不明显。 结论ZDY10 1、SZ2 0 1在一定浓度时 ,能增加α -sAPP的生成 ,二者联合应用效果更强。

Objective To study the effect of single or combined application of ZDY101 and SZ201 on the generation of α-sAPP by HEK293sw cells. Methods The levels of α-sAPP from HEK293sw cells were examined by Western blot and the effects of single or combined application of ZDY101 and SZ201 on generation of α-sAPP were observed from 24h to 48h or 48h to 72h. Results In the time period of 24h to 48h,when ZDY101 or SZ201 was applied separately at 1×10 -5mol/L,the level of α-sAPP was 2.06±0.73 and 2.64±1.21,respectively significantly higher than the DMSO control(1.00).In the time period of 48 to 72h,when the concentration of ZDY101 or SZ201 was 1×10 -5mol/L,the level of α-sAPP was 1.77±0.5 and 2.31±0.62,respectively also significantly higher than the DMSO control(1.00).When the two substances were used in combination,each at 1×10 -5mol/L,the level of α-sAPP was higher than that with single ZDY101 or SZ201 group.However,when the concentrations of both substances were lowered to make the total concentration equal to 1×10 -5mol/L,the elevation effect on α-sAPP was not significant. Conclusion ZDY101and SZ201 promote the generation of α-sAPP when the concentration of the substances reached certain level,combination of ZDY101, and SZ201 is more effective than ZDY101 or SZ201 alone.