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重组E.coli工程菌高密度培养生产人源型胶原蛋白 被引量:66

HIGH DENSITY FERMENTATION OF RECOMBINANT E.coli FOR PRODUCTION OF HUMAN-LIKE COLLAGEN
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摘要 Several technical parameters were studied during the fermentation of recombinant E.coli for the production of collagen-like biopolymer.The effects of dissolved oxygen as well as glucose concentration on fermentation were observed.The OD 600 value could reach 98 when dissolved oxygen was controlled at 50% and glucose around 1%.The production of human-like collagen with a yield of 29.4% was obtained. Several technical parameters were studied during the fermentation of recombinant E.coli for the production of collagen-like biopolymer.The effects of dissolved oxygen as well as glucose concentration on fermentation were observed.The OD 600 value could reach 98 when dissolved oxygen was controlled at 50% and glucose around 1%.The production of human-like collagen with a yield of 29.4% was obtained.
机构地区 西北大学化工系
出处 《化工学报》 EI CAS CSCD 北大核心 2002年第7期752-754,共3页 CIESC Journal
基金 国家教委留学回国人员科研基金 陕西省科技攻关项目(No .2 0 0 0K13 -G5 ) 陕西省教委重大攻关项目 (No .0 0JK -0 0 2 ) 西安市重大创新计划项目 (No.CX2 0 0 0 0 8)资助
关键词 重组E.coli工程菌 高密度发酵 类人胶原蛋白 培养工艺 发酵 recombinant E.coli ,high density fermentation,human-like collagen
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  • 1[1]Ellis DA, Braig F, Ropertsop C, et al. Collagen implant: An early assessment. J Otolaryngol,1984,13(4):267-272.
  • 2[2]Knapp TR, Kaplan EN, Daniels JR, et al. Injectable collagen for soft tissue augmentation. Plast Reconstr Surg,1977,60(3):398-402.
  • 3[3]Knapp T, Luck E, Daniels J, et al. Behavior of solubilized collagen as a bioimplast. J Surg Res,1977,23:96-100.

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