气相色谱法测定芦荟及芦荟干粉中的多糖

Determination of Poly-saccharides in Aloe and Aloe Dry Powder by Gas Chromatography

提取芦荟及芦荟干粉样品中的多糖 ,将多糖水解为单糖之后衍生化反应 ,用气相色谱法进行分析 .实验选择了最佳分离条件 ,以木糖醇为内标物 ,用OV 2 2 5与OS 138为色谱柱固定液 ,使衍生物得到了很好的分离 .结果表明 :未经水解的芦荟中含有少量的单糖以葡萄糖的形式存在 ,水解后的单糖主要为甘露糖和葡萄糖 .这与用乙醇沉淀多糖后测定结果一致 .即芦荟及芦荟干粉中的多糖主要为甘露聚糖和甘露葡聚糖 .

The poly saccharides of aloe and aloe dry powder samples were analyzed by gas chromatography, which using alcohol deposition saccharides of aloe and aloe dry powder, after hydrolyzing and derivativing saccharides. Under optimum conditions, a mixed column of OV 225 and OS 138 was utilized. It attained a better quantitative determinations by the condition of xylitol was internal standard. Experimental results indicated that there was a little glucose in the aloe which had not been degraded; Mannose and glucose were major saccharides in the aloe which had been degraded. The similar result was obtained from carbohydrates which had reacted with alcohol deposition. These analytic results indicated that the saccharides of aloe and aloe dry powder samples were manna polysaccharide and manna glucpolysaccharide.