重组人生长激素对内毒素血症大鼠的治疗作用

Therapeutic effect of recombinant human growth hormone on rat endotoxemia

目的 :观察重组人生长激素 (rhGH)对大鼠内毒素血症的治疗效果。方法 :76只SD大鼠随机分为正常对照组、内毒素血症组 (仅腹腔注射鸵鸟株大肠杆菌 (1 5ml/kg ,1× 1 0 10 cfu/L)及治疗组 (腹腔注射鸵鸟株大肠杆菌后行rhGH肌肉注射 (2 2 5IU/kg/d)。内毒素血症组及治疗组又依观察时点再分为 1天、3天两个亚组。采用放射免疫分析方法测定血浆肿瘤坏死因子 α(TNF α)浓度并观测大鼠血压的变化。结果 :与正常对照组相比 ,无论是内毒素血症组还是治疗组 ,1天时其大鼠血浆TNF α浓度均明显升高 (P <0 .0 5 ) ;虽然两组大鼠血压都降低 (P <0 .0 5 ) ,但治疗组大鼠血压降低幅度明显小于内毒素血症组 (P <0 .0 0 1 )。 3天时 ,内毒素血症组大鼠血浆TNF α浓度降至正常对照水平 ,而治疗组大鼠血浆TNF α浓度却明显低于正常对照组 (P <0 .0 0 1 )及内毒素血症组 3天水平 (P <0 .0 5 )。结论 :rhGH能明显减轻内毒素血症大鼠血压的下降 ,对内毒素血症大鼠具有较理想的治疗效果 ;

Ojbective: To investigate the therapeutic effect of recombinant human growth hormone (rhGH) on rat endotoxemia. Methods: 76 rats were divided into 3 groups randomly: normal control group, treated group and endotoxemia group. The treated group received E. coli (15ml·kg 1 , 1×10 10 cfu·L 1 ), then followed by rhGH injection (2.25IU/kg/d), the endotoxemia group just received E. coli without rhGH. Blood pressure, concentration of tumor necrosis factor α(TNF α) in plasma were determined using radioimmunoassay. Results: On 1st day, Compared with that of normal control group, the levels of plasma TNF α were increased significantly in treated and endotoxemia groups (P<0.05). Blood pressure in treated and endotoxemia rats also decreased, but blood pressure in endotoxemia rats decreased more (P<0.001). On 3rd day, concentration of TNF α in endotoxemia rats returned to the level of normal control group, while the level of plasma TNF α in treated rats lower more than that of endotoxemia (P<0.05) and normal control groups (P<0.001). Conclusion:rhGH can attenuate the decrease of blood pressure of rat endotoxemia and showed a promising therapeutic effect on rat endotoxemia,which may involve the inhibition of the release of TNF α.