不同宫颈组织中nm23-H1表达

Expression of nm23-H1 Gene in Different Cervical Tissues

目的 探讨不同宫颈组织中 nm2 3- H1表达 ,旨在了解 nm2 3- H1在宫颈癌发生发展及预后的作用。方法 采用免疫组化法 HIGH- SABC法检测宫颈鳞状上皮癌 (SCC) 5 0例、低度不典型增生 (CIN 、CIN )及重度不典型增生(CIN )各 15例、正常宫颈鳞状上皮组织 10例中 nm2 3- H1的表达。结果 正常宫颈组织和低度不典型增生中 nm2 3- H1阳性表达率均明显高于 SCC(P<0 .0 5 ) ,CIN 中 nm2 3- H1阳性率明显减少 ,与 SCC相比 ,两者间无统计学差异。 SCC中 nm 2 3- H1的表达与癌细胞分化程度呈负相关 ,与其他参数和预后不相关。结论 nm2 3- H1的低表达在宫颈鳞癌序列发生发展中可能起一定作用 ,但不能作为宫颈鳞癌预后评价指标。

Objective To expore the expression of nm 23-H1 gene and its s ignificance in different cervical tissues.Methods Expressions o f nm23-H1 gene protein in 50 cases with squmous cell carcinoma(SCC),15 with low-gradesqua mous dysplasia of the cervix(CINⅠ?CINⅡ) and high-grade squamous dysplasia of the cervix(CINⅢ) respectively and 10 with normal tissue of the uterine cervix we re examined by immuno-histochemical staining.Results Positive rate of nm23-H 1 protein was significant higher in normal cervica l tissue and low-grade CIN t han that in SCC,and significantly lower in all of the high-grade CIN,However,th ere was no statistically significant difference in nm23-H1 immunoreactivity b etween cases of high-grade CIN and SCC.nm23-H1 gene protein only negatively co rrelated with cell differentiation and there was no statistic relation with othe r clinicopathology parameterd and prognosis.Conclusion These fi ndings suggested that the reduced expression of nm23-H1 might be important in the sequential de velopment of cervical squamous neoplasia,however,it may not be regard as an usef ul marker predicting prognosis of squmous cell carcinoma.