离体人睾丸缺血再灌注损伤模型

A New Model on Isolated Human Testes with Ischemia-Reperfusion Injury

目的 建立离体人睾丸的缺血再灌注 (I/R)损伤模型 ,为研究抗I/R损伤药物的作用及机理提供一实质器官灌注模型。方法 采用 13例捐赠尸睾 ,用 2 5 0ml 0℃～ 4℃高渗枸橼酸盐嘌呤液灌洗后冷存 ,再以 5 0 0ml 3 7℃该液进行再灌注 ,不同时间点取材作组织学及酶组织化学检查。结果 4℃冷缺血 12h开始出现血管内皮细胞肿胀、变圆、空泡样变 ,2 4h内皮细胞变性脱落 ,睾丸曲细精管基膜与生精上皮剥离 ,生精细胞变性脱落 ,间质水肿等 ,病变随冷缺血时间延长而加重。酶组化结果显示 ,单纯冷保存 18h后 ,睾丸组织乳酸脱氢酶 (LDH )活性升高 ,琥珀酸脱氢酶 (SDH )活性2 4h后升高 ,经 3 7℃复温再灌注后损伤明显加重。结论 离体人睾丸可替代人体其它实质器官作为研究I/R损伤的离体灌注模型。

Objective To establish a new model on isolated human cadaver testes with ischemia reperfusion (I/R). Methods Thirteen isolated cadaver testes contributed by 13 persons were preserved under 0℃-4℃ hypothermia and then reperfused under 37℃. Histological and histochemical changes were observed. Results 4℃ cold ischemia in 12 hours induced only trivial swelling and vascular degeneration of endothelial cells (ECs), obvious pathologic changes occurred after 24 hours, including detachment of ECs, separation between basement membrane and seminiferous epithelium, degeneration and detachment of spermatogenous cell and edema of mesenchyme. Injury was worse along with the prolongation of cold preservation time. Changes of LDH and SDH activities were found by histochemical staining. Reperfusion following 6 hours ischemia induced tissue injury and unusual enzyme activity. All changes were more obvious after reperfusion following 12,18,24 or 36 hours cold ischemia.Conclusion This new model on isolated cadaver testes with ischemia reperfusion is successful, it can substitute other solid organs of human beings for I/R injury study.