nm23基因在人胰腺癌中的表达及其意义

Expression and Significance of nm23/NDPK in Pancreatic Adenocarcinomas

目的 探讨nm2 3 /NDPK癌基因在胰腺癌组织中的表达及其临床意义。方法 采用免疫组化SP法 ,对 40例胰腺癌组织及 14例正常胰腺组织进行nm 2 3 /NDPK癌基因检测。结果 40例胰腺癌组织中有 2 6例nm 2 3 /NDPK表达阳性 ,占 65 .0 % ;14例正常胰腺组织中仅 4例nm 2 3 /NDPK表达阳性 ,占 2 8.6% ,两者差异有显著性意义 (P<0 .0 5 )。低分化腺癌nm2 3 /NDPK表达阳性率为 90 .9% ,明显高于高分化胰腺癌 (2 5 .0 % ) ,P<0 .0 5。nm2 3 /NDPK表达阳性率在淋巴结转移阳性者为 71.4% (10 /14 ) ,明显高于淋巴结转移阴性者 (3 1.6% ,6/19) ,P<0 .0 5。提示胰腺癌nm 2 3 /NDPK表达与淋巴结转移及肿瘤侵袭性呈正相关 ,与癌组织的分化程度呈负相关。结论 nm 2 3 /NDPK表达可作为胰腺癌恶性程度及预后不良的生物学指标之一。

Objective To study the expression and clinic significance of nm23 gene (product of uncleoside ciphosphate kinase) in the pancreatic adenocarcinoma tissues. Methods S P immunohistochemical method was used to examine the expression of nm23/NDPK in 40 pancreatic adenocarcinoma and 14 normal pancreas tissues.Results Twenty six of 40(65%) pancreatic adenocarcinoma showed strong immunoreactivity for NDP kinase, whereas 4 of 14 (28.5%) normal pancreatic tissues showed weak immunoreactivity. Significant difference was found between the two groups ( P <0.05). The nm23/NDPK expression levels in pancreatic adenocarcinoma of lower differentiation was higher than those in pancreatic adenocarcinoma of higher differentiation (10/11,90.9%; 2/8, 25%; P <0.05). Positive staining was associated with higher incidence of lymph node metastasis (10/14, 71.4%) than negative staining (6/19,31.5%, P <0.05). These results suggested that nm23/NDPK expression was positively associated with lymph node metastasis and aggressiveness. They also suggested that nm23/NDPK expression had negative correlation with the extent of histologic differentiation. Conclusion nm23/NDPK can serve as a marker for malignant potentiality and indicate the prognosis of pancreatic adenocarcinoma.