电针对局灶性脑缺血大鼠脑源性神经营养因子影响的研究

Effect of Electroacupuncture on Brain-derived Neurotrophic Factor in Rats with Focal Brain Ischemia

目的 :研究电针督脉经穴“大椎”、“百会”对局灶性脑缺血大鼠脑源性神经营养因子的影响。方法 :凝闭大鼠一侧大脑中动脉 1 0hr后致局灶性脑缺血 ,采用免疫组化染色S P法进行观察。结果 :电针治疗能增强局灶性脑缺血大鼠脑组织脑源性神经营养因子的表达 ,从而阻止神经细胞内Ca2 +超载 ,稳定细胞内环境。结论 :电针对缺血性脑损伤具有一定的保护作用 ,为临床针灸治疗缺血性脑血管疾病奠定了理论基础。

Objective:To study the effect of electroacupuncture (EA) of 'Dazhui' (GV 14) and 'Baihui' (GV 20) on brain-derived neurotrophic factor (BDNF) expression in brain tissues of cerebral ischemia rats for analyzing the mechanism of acupuncture in preventing the ischemic cerebral neurons from injury. Methods: Local cerebral ischemia model was established by occlusion of the middle cerebral artery (MACo) of the rat. 24 Wistar rats were randomly and evenly divided into sham-operation group, cerebral ischemia group and EA+ischemia group. 'Dazhui' (GV 14) and 'Baihui' (GV 20) were punctured and stimulated electrically by setting the stimulating parameters as frequency of 5～ 10 Hz, sparse-dense waves, strength of 35 V and duration of 30 min. EA was given once again following 5-hours'cerebral ischemia. The rats' brain tissues were cut into sections (6 μm thick) that were stained with immunochistochemical method. Results: In sham-operation group, the number of positive BDNF-immunoreaction cells was smaller and the reaction density was lighter. In cerebral ischemia group, BDNF expression neurons increased remarkably in quantity in comparison with sham-operation group. In cerebral ischemia + EA group, BDNF expression cells increased considerably in quantity and in density compared with control group and cerebral ischemia group (P<0.01). That means EA has a protective action on ischemia cerebral injury. Conclusion: EA can potentiate BDNF expression in ischemic cerebral tissues, which maybe contribute to the effect of acupuncture in relieving ischemic injury of the cerebral neurons.