电针合用抗抑郁药能明显减少小鼠强迫游泳实验中的静止时间

Electroacupuncture Combined with Antidepressive Drugs Can Reduce the Immobility Time during the Forced Swimming Test in Mice

目的 :本实验采用小鼠强迫游泳实验的方法 ,观察针刺合用抗抑郁药后小鼠在水中的静止时间 ,以此来分析抑郁程度的改善情况。方法 :实验分下列各组 :①生理盐水组 ;②电针加生理盐水组 ;③药物组 ,分别观察了氯丙咪嗪 (氯米帕明 2 .5mg/kg、5mg/kg、1 0mg/kg、2 0mg/kg、60mg/kg)、芬氟明 ( 1 .5mg/kg、3mg/kg、1 2mg/kg)和吗氯贝胺 ( 1 0mg/kg、2 0mg/kg、40mg/kg)等三种药物的作用 ;④上述药物加电针组。结果 :电针能使小鼠在水中静止的时间显著减少 ,腹腔注射 (ip)上述三种药物也能减少小鼠在水中静止的时间 ,并表现出一定的剂量依赖关系。在电针加药组中 ,分别选用三种药物的小剂量 (抗抑郁效果很弱或几乎无效 )与电针合用。氯丙咪嗪( 2 .5mg/kg)、芬氟明 ( 1 .5mg/kg)与电针合用后 ,小鼠在水中静止时间与单用药物组或单电针组相比显著减少。而吗氯贝胺 ( 1 0mg/kg)与电针合用虽也有这种趋势 ,但统计学上尚未达到显著性差异。结论 :电针具有抗抑郁作用 。

Objective: In the present study, the effect of electroacupuncture(EA) combined with drugs on the depression was observed by a forced swimming test. Methods: Male Kunming mice were randomly divided into: ① normal saline(NS, n=10) group, ② NS plus EA group (n=10), ③ drug groups--chlorimipramine(2.5 mg/kg, 5 mg/kg,10 mg/kg,20 mg/kg,60 mg/kg), fenfluramine(1.5 mg/kg,3 mg/kg,12 mg/kg) and moclobemide(10 mg/kg,20 mg/kg,40 mg/kg), ④ drug plus EA groups (chlorimipramine 2.5 mg/kg+EA, fenfluramine 1.5 mg/kg + EA, and moclobemide 10 mg/kg + EA). The number of each one-dosage subgroup of each drug in drug groups and drug plus EA groups was 10. EA (sparse waves 4 Hz, series duration 2.5 sec, dense waves 20 Hz, series duration 5 sec, strength≤1 mA) was applied to 'Baihui'(GV 20) and Anmian (EX-HN 16) for 40 min. These 3 types of drugs were given by intraperitoneal injection. Results: In comparison with NS group, the duration of immobility (DI) during 5 min test in EA plus NS group was decreased significantly (P<0.01). Compared with that of NS group, the values of DI in chlorimipramine (2.5～ 60 mg/kg, slight dose-dependent tendency), fenfluramine (3～6 mg/kg, no dose-dependent tendency) and moclobemide (10～40 mg/kg, marked dose-effect dependent relationship, r=0.998) groups were also reduced pronouncedly (P<0.01). When the low dose of chlorimipramine (2.5 mg/kg) and fenfluramine (1.5 mg/kg), which hardly had antidepressant effect, were combined with EA, DI values were decreased significantly more than those of either EA plus NS group or drug groups (P< 0.01). The DI of Moclobemide(10 mg/kg) plus EA was smaller remarkably than that of Moclobemide (10 mg/kg, P<0.01), but had no statistical difference compared with that of EA+NS group (P>0.05). Conclusion: EA has an antidepressant effect and EA plus some antidepressants in low doses can potentiate the action of EA further, suggesting that EA plus antidepressant may be a good way for treating depression.