山东地区汉坦病毒重要基因序列进化关系的研究

The important genetic phylogenetic analysis of hantaviruses in shandong

目的 :研究山东地区汉坦病毒的基因型分布。方法 :应用RT -PCR法和直接核酸测序法 ,对来源于山东省 11个肾综合征出血热流行地区的患者中获得的 31份汉坦病毒部分M基因片段进行了扩增和测序。结果 :核苷酸序列的同源性及系统发生树分析表明 ,山东地区SEO型汉坦病毒至少存在 4个不同亚型 ,HTN型汉坦病毒至少发现有 4个分支 ,不同HTN型汉坦病毒亚型间的核苷酸序列的差异达 13.0 %～2 2 .7% ,而不同SEO型汉坦病毒分支间相对保守 ,差异仅 5 .0 %～ 7.7%。结论 :山东地区存在汉坦病毒的不同的基因亚型 ,这一发现将促进汉坦病毒流行规律。

Objective: To find out the distribution of Hantaviruses genotypes in Shandong. Methods: The partial M segments of 31 Hantaviruses isolated from patients with HFRS in 11 endemic regions of Shandong were amplified by RT-PCR and sequenced by direct sequencing . Results: Phylogenetic analysis suggested at least 4 different subtypes belonging to a novel subgenotype of SEO existed in Shandong and HTN virus branched into 4 differents clades. The differences of partial M nucleotide sequence between the subtypes of HTN viruses (13.0%～22.7%) were higher than those among subtypes of SEO viruses (5.0%～7.7%). Conclusion: Different subgenotypes of hantaviruses are found to exist in Shandong according to the genotype analysis of this study,which prouides an advantageoud basis for the further study of epidemic regularity, pathogenicity and vaccine.