人工角膜支架材料的实验研究

The experimental study of biological response of synthetic materials used for keratoprosthesis

目的 :观察 3种人工角膜支架材料在动物体内的生物学反应。方法 :将多孔材料、Polyester材料和膨体聚四氟乙烯三种材料切成直径 6mm的圆盘状。分为处理组和对照组。处理组的材料经氩等离子体处理。把 4 2只新西兰大白兔分为 7组 ,每组 6只兔 ,3组为实验组 ,3组为对照组 ,1组为手术对照组。将盘状支架材料手术植入角膜板层囊袋内。术后第 4 2、84天取角膜进行角膜组织学及生物化学分析。结果 :材料处理组角膜水肿明显减轻 ,8周后 3种材料中角膜水肿均消失。多孔材料和polyester材料植入后新生血管出现延迟 ,范围较小 ,炎细胞浸润也明显减少。术后 6周纤维细胞迁徙材料内 ,术后 12周空腔填充大量纤维细胞 ,材料的厚度明显增加。同时葡萄糖氨基聚糖分析发现 ,KeratinSulfate (KS)含量降低 ,DermatinSul fate(DS)含量增高 ,并有大分子葡萄糖氨基聚糖出现。材料处理与否不影响胶原蛋白及葡萄糖氨基聚糖的沉积。结论 :支架材料经氩等离子体处理可明显减轻角膜炎症反应 ,增加材料内间质蛋白的沉积 。

Objective: To study and compare the biological response to the corneal stroma of three porous materials treated with argon plasma. Methods: Three porofus materials were chosen: a melt blown-microfiber web of polybutylene : polyproplene (80:20); polyester spun laced fabric; and an expanded polytetrafluoroethylene. Fifty percent of each material was modified through argon plasma treatment. Discs with a diameter of 6mm were inserted into interlamellar stromal pockets and observed for a period of 12 weeks with an examination at weekly interval. Results: Pretrement of the discs reduced inflammation and corneal edema. The neovascularization appeared later in the treated materials. Conclusion: Argon plasma treatment increases the biocompatibility of all materials. Fibroplasia occurred most rapidly into the polyester discs but was accompanied by a large number of inflammatory cells.