平菇多糖清除O_2^-及对红细胞膜自由基氧化的影响

Effects of Pleurotus ostreatus Polysaccharide on Eliminating Super O_2^- and Peroxidation of Free Radical of Erythrocyte Membrane

平菇经热水抽提 ,Sevag法去蛋白后 ,用乙醇沉淀 ,得平菇粗糖。上SephadexG - 10 0层析 ,可得精制多糖。在体外反应产生O2 -,此体系中 ,加入不同浓度的平菇多糖。平菇多糖在较低浓度时 (<2 0 0mg/L)具有清除O2 -作用 ,而在较高浓度 (>2 0 0mg/L)时 ,作用不明显。同时 ,用荧光法研究平菇多糖对小鼠红细胞脂质过氧化的影响 。

Polysaccharide(PS) was extracted from Pleurotus ostreafus and purified by ethanol fraction and SephadexG-100 chromatography. PS at lower concentrations could eliminate super O 2 - (<200mg/L), but could not at higher concentrations (>200mg/L). The result also showed that PS could Inhibit lipid peroxidation of mouse erythrocyte membrane.