施万细胞条件培养基对大鼠骨髓间质细胞的诱导分化作用

INDUCTION OF DIFFERENTIATION IN BONE MARROW STROMAL CELLS OF RATS BY CONDITIONED MEDIUM OF SCHWANN CELLS

目的 探讨施万细胞条件培养基对大鼠骨髓间质细胞的诱导分化作用。方法 从大鼠骨髓中分离培养间质细胞并传至第 6代 ,诱导前 2 4h加 1μg·L-1碱性成纤维生长因子 (bFGF)入培养液中以促分裂 ,再以施万细胞条件培养基作诱导剂 ,观察细胞形态的变化 ,并采用免疫组织化学法对诱导后一周的细胞进行Map 2及NSE、GFAP表达的检测。结果 诱导后 48小时间质细胞在形态上表现为神经元样 ,神经元样细胞呈Map 2及NSE阳性 ,而GFAP显阴性。结论 施万细胞的上清液能诱导骨髓间质细胞分化为神经元样细胞。

Objective To study the differentiation-inducing effects of the conditioned medium of Schwann cells on bone marrow stromal cells (BMSCs) of rats. Method The BMSCs from rats were isolated and cultured for 6 passages. They were pretreated for 24 h in DMEM containing 20% FBS (v/v) and 1 μg·L -1 bFGF. Then the medium was replaced with the conditioned culture medium of Schwann cells for induction. The morphological changes of the cells were observed. on 4 d after induction, the induced cells were stained immunocytochemically with Map-2, neuron-specific enolase (NSE) and glial fibrillary acidic protein (GFAP) antibodies.Result Morphological changes of the stromall cells were noted 48 hours after induction. One week after induction, the induced cells were Map-2 and NSE positive, and GFAP negative.Conclusion The results show that the bone marrow stromal cells can be induced to differentiate into neuron-like cells by the conditioned medium of Schwann cells.