Dig标记探针原位杂交检测MSG-肝再生-大鼠下丘脑弓状核TGF-β_1mRNA

THE EXPRESSION OF TGF-β_1 mRNA IN THE HYPOTHALAMIC ARN OF RAT WITH MSG-LIVER REGENERATION USING IN SITU HYBRIDIZATION

目的 通过建立MSG 肝再生 大鼠模型 ,检测MSG 肝再生 大鼠下丘脑弓状核 (ARN)TGF β1mRNA的表达与神经内分泌免疫网络的关系。方法 用肝大部分切除MSG 大鼠的方法建立MSG 肝再生 大鼠模型 ,原位杂交技术检测MSG 肝再生 大鼠下丘脑弓状核 (ARN)TGF β1mRNA的表达。结果 发现MSG -肝再生 -大鼠ARNTGF - β1mRNA的表达显著上调 ,切除 11天达 2 0 9± 1 6 ,与生理盐水组 (11 1± 1 2 )和MSG -大鼠假手术组 (15 2± 1 1)比较 ,差异显著 (P <0 0 1)。结论 MSG -肝再生 -大鼠神经 -内分泌 -免疫网络功能紊乱与其ARNTGF - β1mRNA表达失调密切相关。

Objective Through The model of liver regeneration of TGF-β 1 mRNA and the relation with the meuroendocrino-immunological network.Method The mode of liver regeneration following partial hepatectomy in the rats treated with monosodium glatamate (MSG). The expression of TGF-β 1mRNA in the hypothalamic arcuate nucleus (ARN) was detected with the aid of in situ hybridization using Dig-labelled probe. Result The expression of TGF-β 1 mRNA in the hypothalamic ARN of the rats treated was higher (20.9±1.6 ) than that of the normal (11.1±1.2 ) and the sham-operated rats (15.2±1.2) on the 11 thday . Conclusion The results suggested that the higher TGF-β 1mRAN expression in the hypothalamic ARN may play some pathophysiological role in the pathogenesis of disorders in the neuroendocrino-immunological network (NEIN) of rats with MSG-liver regeneration. ;