氮磷饥饿诱导的水稻糖转运体基因的cDNA克隆和鉴定(英文)

cDNA Cloning and Characterization of a Gene for Sugar Transporter Inducible by Nitrogen and Phosphorus Starvation in Rice (Oryza sativa L.)

运用快速扣除杂交 (RaSH)方法构建了水稻氮饥饿诱导的cDNA文库。从该文库获得了一个cDNA克隆OsNSI1 (Oryzasativanitrogenstarva tion inducible 1 )。该全长cDNA编码 5 77个氨基酸 ,蛋白分子量为 6 1 .2kD。推测得出的氨基酸序列与其他物种的糖转运体有很高的同源性。水合性分析表明OsNSI1包含有 1 2个跨膜区域和一个中心亲水环。这些数据提示OsNSI1是一个糖转运体蛋白。Southern印迹分析表明OsNSI1是一个单拷贝基因。Northern印迹分析表明OsNSI1主要在叶及根中表达 ,氮。

A cDNA library from nitrogen-starved rice (Oryza sativa L.) was constructed using RaSH procedure. A cDNA clone, OsNSI1 (Oryza sativa nitrogen starvation-inducible 1) was isolated from this library. The full-length cDNA encodes a protein of 577 amino acids with a predicted molecular weight of 61.2 kD. The deduced amino acid sequence of OsNSI1 shows high homology to sugar transporters from other species. Hydropathy analysis revealed that OsNSI1 protein composed of 12 transmembrane domains and a central hydrophilic loop. These data suggest that OsNSI1 encodes a sugar transporter. Genomic Southern blot analysis showed that OsNSI1 may be a single-copy gene in rice genome. Northern blot analysis revealed that OsNSI1 was mainly expressed in leaves and roots, and upregulated by N- and P-starvation.