死胎脾脏组织中HBcAg检测的临床和病理研究

Clinical and pathological study on the expression of HBcAg in the spleen tissues of the dead fetus

目的 :观察乙型肝炎病毒 (HBV)通过产妇传播在胎儿脾脏组织中表达的情况。方法 :采集 40例乙型肝炎产妇产下的死胎 ,常规尸检 ,取脾脏组织 ,SP法检测HBcAg ;回顾分析产妇产前静脉血HBV的检测结果 ;取百分率行u检验。结果 :HBcAg阳性颗粒在死胎脾脏组织的白髓、边缘区、红髓中的淋巴细胞和巨噬细胞质、血窦、血管腔内呈点、灶状分布 ,脾脏淋巴细胞和巨噬细胞核不着色。HBV呈大三阳的产妇较HBV呈单项阳性、小三阳的产妇分娩的死胎脾脏组织中HBcAg阳性率明显升高 ,但彼此比较无显著性差异 (P >0 .0 5 )。结论 :死胎脾脏组织中HBcAg阳性率与产妇HBV静脉中HBV标志无关。

Objective To study the expression of HBcAg in the spleen tissue of the dead fetus infected by hepatitis virus B(HBV) through maternal fetal transmission.Methods 40 cases of spleen tissues of dead fetus in the puerperal delivery with HBV were detected for HBcAg by immunocytochemistry(SP).And the detection on HBV in the mother veins before delivery were considered.Results HBcAg were found positive both single or inclusters form distributed in the plasma of the lymphocyte and the macrophagocyte in the white pulp,marginal zone,red pulp and spleen vessels,but not in the spleen cell nuclei.The HBcAg positive rate is obviously higher in the pleen tissues of the dead fetus delivered by mother with HBV for only one HBVM + than HBsAg + HBeAb + HBcAb +.But there is not much statistical difference in comparison( P >0.005).Conclusions The positive rate of HBVM in the spleen was not correlated with the HBVM in the pregnant woman veins.HBV maybe copy in the spleen tissues.