雀舌黄杨(Buxus harlandii)愈伤组织遗传转化的研究

Studies on genetic transformation of calli of Buxus harlandii.

利用处在对数生长期的根癌农杆菌Bo Ss和T37菌株和雀舌黄杨愈伤组织共培养后,在无激素MS培养基上获得了转化愈伤组织,经测定转化的愈伤组织内存在相应的冠瘿碱,结果证明T-DNA编码的激素合成酶基因和冠瘿碱合成酶基因在转化愈伤组织内实现了转移和表达。

The calli of Buxus harlandii were co-cultivated with Agrobacterium Tumefaciens strains B6 S3 and T37 which were at longarithmic growth stage.Transformed calli were obtained on MS medium lacking hormones.In the transformed calli measured,there were the corresponding opines,The results demonstrated that the hormone synthase gene and opine synthase gene coded by T-DNA were transferred and expressed in transformed,calli.