水稻亚种间杂种一代部分雄性不育的细胞学研究

Cytological studies on partial male sterility of F_1 hybrids between subspecies in Rice. Acta Agriculturae Universitatis Zhejiangensis,

水稻亚种间广亲和组合 CPSLO 17×IR_(26)和不亲和组合 7627 B×IR_(26)杂种一代部分雄性不育的细胞学研究阐明在四分体时期以前一般花药发育均正常,单核期后 7627 B×IR_(26)杂种F_1部分花粉败育。TTC染色反应显示 CPSLO 17×IR_(26)组合比 7627 B×IR_(26)组合花粉脱 H 酶活性高。此外,CPSLO 17×IR_(26)F_1花药在位于药隔两侧的药室间凹陷部位底部的表皮细胞下形成两个发达的“药室间组织间隙”,这对花药开裂十分有利。荧光染色法看出两个不同杂种柱头上萌发的花粉粒数最有显著差异。

The results of (he cytological studies of partial male sterility in F1 hybrid between subspecies crosses of widely compatible CPSLO 17×IR6 and pooly compatible 7627 B×IR26 in rice are described. Anther development was usually normal before tetrad stage. The partial pollen abortion in F1 hybrid of 7627 B×IR26 occurred after uni-nucleate stage. TTC stained reaction indicated that dehydrogenase activity in F1 pollen grains of cross between CPSLO 17 and IR26 was higher than that of cross between 7627 B and IR26. In addition, in the F1 anther fo cross between CPSLO 17 and IR26,between two anther chambers that lay at the connective bilateral edge two 'tissue spaces between two anther chambers' were formed beneath the epidermal cells at the bottom of the concave. This was very favarable for the opening of the anther. By using fluorescence staining method, there were significantly differences In the number of germinated grains on the stigma between two hyorids.