摘要
目的研究胞嘧啶脱氨酶基因 (CD)联合化疗药物对结肠癌的治疗作用。方法以重组腺病毒AdCMVCD为载体转移CD基因 ,在体外研究病毒感染对顺铂 (CDDP)、丝裂霉素 (MMC)敏感性的影响 ,化疗药物对AdCMVCD/ 5 FC直接杀伤作用及“旁观者效应”的影响。结果病毒感染并不影响Lovo细胞对CDDP、MMC的敏感性 ;低剂量CDDP、MMC即可明显增强AdCMVCD/ 5 FC系统的直接杀瘤作用 ,5 FC的IC50 明显降低 (P <0 0 5 ) ,并且显著增强AdCMVCD / 5 FC系统的“旁观者效应”。结论化疗药物可以增强自杀基因系统对结肠癌的治疗作用 。
ObjectiveTo evaluate the effect of combined cytosine deaminase(CD) gene therapy and chemical reagents on colon cancer cell line.MethodsLovo cells transducted with CD gene in a recombinant adenovirus AdCMVCD vector were treated with 5 fluorocytosine(5 FC) and ciplatin(CDDP) or mitomycine C(MMC). Cytotoxic effect was assayed with MTT method. ResultsLow dose of CDDP or MMC reduces the IC 50 of 5 FC significantly on Lovo cells transducted with CD gene and enhances the bystander effect of AdCMVCD/5 FC system.ConclusionCombined therapy with CD/5 FC system and chemical reagents may have a potential application in the treatment of colon cancer.
出处
《中华普通外科杂志》
CSCD
北大核心
2002年第7期404-406,共3页
Chinese Journal of General Surgery
基金
南京医科大学创新基金 (Cx990 5 )
