重组人粒细胞巨噬细胞集落刺激因子比色测定中最适波长的研究

The Propericitate Wavelergths Determination of the Recombination Human Granulocyte-Macrophage Colony Stimulating Factor by Colorimetric Determination

目的研究重组人粒细胞巨噬细胞集落刺激因子比色测定中的最适波长。方法采用UV-2001紫外分光光度计在0～700nm波长处测定其吸收度,绘制标准曲线。结果该法测定样品在595nm处有最大吸收度,在695处无吸收。结论重组人粒细胞巨噬细胞集落刺激因子比色测定中的最适波长为595nm。

Purpose Study on the optimum wavelength determination of the Recombinant Hu- man Granulocyte Macrophage Colony Stimulating Factor by colorimetric method. Method Detect the absorbance at 0～700nm to draw the standand curve using UV-2001 ultra spectopho- tometer. Result There are max absorbance at 595nm, While there are no absorbance at 695nm for the sample. Conclusions The optimum wavelength for detecting the Recombinant Human Granu- longte Macrophage Clong stimulating Factor is 595nm.