胰高血糖素样肽-2对严重烧伤大鼠肠黏膜屏障功能的影响

Effects of glucagon-like peptide-2 on intestinal barrier function in severely burned rats

目的:研究外源性GLP-2对严重烧伤大鼠肠黏膜屏障功能的影响,探索保护烧伤后肠黏膜屏障功能的新措施。方法:以30％体表面积Ⅲ度烧伤大鼠为模型,随机分为GLP-2治疗组和单纯烧伤组,分别经腹腔注射GLP-2(每日200μg/kg)和PBS,于烧伤后0、1、3、5、7d进行肝、脾及门静脉血细菌培养,分别观察门静脉血浆内毒素含量、血清DAO活性及D-乳酸含量的变化。结果:GLP-2治疗组烧伤后3d时脏器细菌总检出率为0,明显低于单纯烧伤组的46.7％(P<0.05);5d时肝、脾和脏器细菌总检出率均为0,明显低于单纯烧伤组(分别为80％,100％和66.7％)。在烧伤后5d时,GLP-2治疗组门静脉血浆内毒素含量,血清DAO活性及D-乳酸含量分别为130.8±33.7EU/L,1779.3±824.2U/L,10.302±1.199μg/mL,均明显低于单纯烧伤组(分别为205.2±57.4EU /L,3336.7±1325.1U/L,13.369±2.199μg/mL)。在烧伤后7d时,GLP-2治疗组门静脉血浆内毒素含量,血清DAO活性及D-乳酸含量分别为127.5±39.0EU/L,1448.6±306.4U/L,9.624±0.799μg/mL,均明显低于单纯烧伤组(分别为157.1±43.2EU/L,2727.3±1002.6U/L,12.651±2.206μg/mL)。结论:应用外源性GLP-2能减轻严重烧伤后肠黏膜损伤,保护肠黏膜屏障功能,对严重烧伤后肠道细菌/内毒素移位有一定的防治作用。

AIM: To investigate the effects of exogenous glucagon-like peptide-2 (GLP-2) on intestinal barrier function in severely burned rats, so as to explore new measure to protect intestinal barrier function in burns. METHODS: Total 45 Wistar rats (200±20g) inflicted with 30% TBSA full thickness burns were randomly divided into GLP-2 treatment group and simple burn group. Rats in each group were peritoneally injected with GLP-2 at a dose of 200μg/kg per day and PBS respectively. On postburn day (PBD) 0, 1, 3, 5 and 7, bacterial cultures were carried out in liver, spleen and portal vein blood. Meanwhile, endotoxin content in portal vein plasma, serum diamine oxidase (DAO) activity and D-lactic acid content were also monitored. RESULTS: Compared with simple burn group, positive culture rates decreased obviously in total tested organs on PBD3 in GLP-2 treatment group (0 vs 46.7%). On PBDS, positive culture rates in liver, spleen, total tested organs in GLP-2 treatment group were significantly lower than those in simple burn group (0 vs 80%, 100%, 66.7% respectively). Endotoxin content in portal vein plasma, serum DAO activity and D-lactic acid content in GLP-2 treatment group were significantly lower than those in simple burn group on PBD5 (130.8±33.7EU/L vs 205.2±57.4EU/L, 1779.3±824.2U/L vs 3336.7±1325.1U/L, 10.302±1.199μg/mL vs 13.369±2.199μg/mL respectively). On PBD7, endotoxin content in portal vein plasma, serum DAO activity and D-lactic acid content in GLP-2 treatment group were 127.5±39.0EU/L, 1448.6±306.4U/L, 9.624±0.799μg/mL respectively, which were obviously lower than those in simple burn group (157.1±43.2EU/L, 2727.3±1002.6U/L, 12.651±2.206μg/mL). CONCLUSIONS: Exogenous GLP-2 attenuates intestinal mucosa damage and protects intestinal barrier function, which may be beneficial to the prevention and treatment of bacterial or endotoxin translocation in severe burns.