烧伤软膏对动物烧伤创面愈合的影响

The effects of burn unguent on deep partial thickness burn wound healing in rabbits

家兔用 95℃热水制成深Ⅱ度烫伤模型 ,创面外用烧伤软膏 ,观察其对家兔深Ⅱ度烫伤早期创面病理变化和创面愈合的影响 ,结果创面外用烧伤软膏可以降低烧伤创面水肿程度 ,增加血浆AT Ⅲ活性 ,增加烧伤创面中FDP含量及羟脯氨酸含量 ,促使Ⅰ /Ⅲ型胶原比例降低 ,减轻烧伤创面早期加深 ,缩短家兔深Ⅱ度烫伤创面愈合时间。表明烧伤软膏具有促进家兔深Ⅱ度烫伤创面愈合时间。

Objective: To observe the effects of burn unguent on deep partial thickness burn wound during early stage and the mechanism of its healing process in rabbits. Methods: The blood samples were taken for measuring antithrombin Ⅲ (AT Ⅲ) activity. The biopsies of burn wound were taken for measuring water content, AT Ⅲ activity, fibrin degradation products (FDP), hydroxyproline and the ratio of collagen Ⅰ/Ⅲ and dermal cell cycle. The time for epithelization of buen wound was also determined. Results: After topical application of burn unguent to deep partial thickness burn wound in rabbits, the water content of the wound decreased. The elevels of FDP and hydroxyproline were elevated, the ratio collagen Ⅰ/Ⅲ decreased, the AT Ⅲ activity in plasma enhanced. The progressive tissue damage in the wound during early stage was alleviated and the time for epithelization of the wound was shorter than that of burn controls. Conclusion: It is suggested that topical application of burn unguent to deep partial thickness burn wound in rabbits may alleviate the progressive tissue damage during early stage and promate wound healing.