胰蛋白酶加乙二胺四乙酸钠溶解草酸钙结石实验研究

Experimental study on dissolution of calcium oxalate calculi with irrigating solutions in vitro

目的 :探讨胰蛋白酶与乙二胺四乙酸钠 (ethylenediaminetraaceticacid ,EDTA)合用溶解草酸钙结石的效果。方法 :用自制玻璃肾盂灌注模型 ,以胰蛋白酶作为基质溶解剂与EDTA合用灌注溶解草酸钙结石 ,用原子吸收光谱法测定溶石剂中钙离子浓度 ,扫描电镜观察结石表面结构的变化 ,并灌注家兔膀胱 ,观察毒性反应。结果 :胰蛋白酶明显增强EDTA溶解草酸钙结石的效果 ,减轻EDTA引起家兔膀胱粘膜的炎性反应。结论 :胰蛋白酶可以用作基质溶解剂 ,加速EDTA对草酸钙结石的溶解 ,并减轻EDTA引起的炎性反应。

Aim: To evaluate the dissolution of calcium oxalate calculi with trypsin and ethylenediamine traacetic acid (EDTA). Methods: In the self-made glass renal cavity mold, calcium oxalate calculi were dissolved with EDTA and trypsin for matrix solvent. The calcium of irrigating solution was determined by atomic absorption spectrophotomatry, and the surface of irrigated calculi were observed by scanning electron microscopy. The pathological changes of bladder mucosae were observed after the rabbit bladders were irrigated. Results: Trypsin could promote EDTA to dissolve calculi and reduce damage to the mucosa caused by EDTA. Conclusion: Trypsin as matrix solvent can speed up the dissolution of EDTA to calculi and reduce the damage of mucosa.