摘要
目的 通过对氨基苯甲酸 (para aminobenzoicacid ,PABA)对牙龈卟啉单胞菌生长代谢的影响 ,研究血链球菌在龈下菌斑生态平衡中的作用。方法 在培养基中加入不同浓度的PABA ,对牙龈卟啉单胞菌行常规厌氧培养 ,测定培养后的细菌浓度吸光度 (A)值和胰酶样蛋白酶活性 ,并用扫描电镜观察菌细胞形态。结果 PABA能促进牙龈卟啉单胞菌的生长和胰酶样蛋白酶的活性 ,当PABA浓度为 1mg/L时 ,其促进作用最强 ,随着PABA浓度的升高 ,促进作用降低 ,当PABA浓度为 10 0mg/L时 ,无明显的促进作用。PABA还能对菌细胞的形态和粘附产生影响。结论 PABA能影响牙龈卟啉单胞菌的生长代谢 ,提示血链球菌可能对龈下菌斑的微生态平衡具有调节作用。
Objective To study the effect of para aminobenzoic acid(PABA ) on the growth and metabolism of Porphyromonas gingivalis (P.g) . Methods After adding different concentrations of PABA into the medium, anaerobic technique was applied to culture P g . The products′ A value and action of TLP was assayed, and P g grew in the medium was observed by a scanning electron microscope. Results PABA promoted the growth of P g and action of TLP, which would reach the highest level when PABA was 1 mg/L, and would decrease with the increasing of concentration of PABA. When the concentration arrived at 100 mg/L, PABA had no effect on them. In the mean time, PABA had effect on the form and adherence of P g . When the concentration was 1 mg/L and 100 mg/L, this effect was strong, but as the concentration was 10 mg/L, the effect disappeared. Conclusions PABA influences the growth and metabolism of P g , which indicate that Streptococcus sanguis has regulative effect on the microecology of subgingival plaque.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2002年第4期275-277,I006,共4页
Chinese Journal of Stomatology
基金
国家自然科学基金资助项目 (3 9670 785 )