局部应用二磷酸盐对鼠正畸牙移动影响的研究

The effects of local administration of Zoledronate solution on the tooth movement and periodontal ligament

目的 探讨局部注射Zoledronate溶液对鼠正畸牙齿移动距离与牙周组织形态的影响。方法 选用 4 2只雄性Wistar大鼠 ,牵引其上颌第一磨牙近中移动。实验中分别将Zoledronate溶液及生理盐水注射入实验组 (左侧 )及对照组大鼠 (双侧 )上颌第一磨牙腭侧的粘骨膜下。注射于实验前 3d开始 ,共进行 9次 ,每 3d一次。分别在加力 0、3、7、14、2 1d后记录上颌第一磨牙移动距离 ,组织HE染色后 ,观察牙周组织形态学的改变。结果 ①实验组大鼠牙齿移动距离明显低于对照组。②实验组大鼠压力侧破骨细胞数在实验全过程中均低于对照组 ,而根分叉区破牙骨质细胞数除加力 14d外 ,2组差异无显著性。③实验过程中Zoledronate溶液对破骨细胞和破牙骨质细胞以外的细胞作用不明显。结论 Zoledronate能有效地抑制支抗牙移动 ,减少压力侧牙槽骨表面破骨细胞数。

Objective To investigate the effects of local administration of Zoledronate solution on the tooth movement and periodontal ligament. Methods Orthodontic tooth movement of upper first molar was performed in 42 rats with coil spring. Zoledronate solution was injected into the palatal submucosal area adjacent to the left upper first molar in experimental group 3 days prior to the use of the appliance. In control group, same amount of 0 9% NaCl solution was injected into the palatal submucosal area adjacent to the left and right upper first molar. The injection was applied every third day. The application of mesial force lasted 0 3 ?7?14?21days respectively. After the rats were sacrificed, the distance of tooth movement was measured. Sections were stained and then observed with microscope. Results 1. The distance of tooth movement in the experimental group was significantly smaller than that in the control group. 2. The number of osteoclast on the pressure side in the experiment group was significantly smaller than that in the control group through the experimental period, but there was no distinct difference between experimental group and control group (except for 14 days) for the number of odontoclast in interradicular area. 3. The osteoclasts and odontoclasts were the main target cell of Zoledronate in periodontal tissue. Conclusions Zoledronate may be a useful agent for anchorage control and reducing the number of osteoclast on pressure side of alveolar bone.