激素性骨坏死骨质丢失与骨保护蛋白表达的相关研究

The relationship between bone-loss of steroid-induced osteonecrosis and local expression of OPG/OCIF

目的 探讨在激素性骨坏死的病程中 ,股骨不同部位骨保护蛋白 /破骨细胞形成抑制因子 (OPG/OCIF)的表达情况 ,与局部骨质丢失是否存在着相关性。 方法 31只新西兰白兔复制Yamamoto激素性骨坏死模型 ,内毒素和甲基强地松龙给药结束 3d后存活 2 4只 ;随机选 4只作为对照组 ,其余随机分为 4个不同时间组 ,每组 5只 ;按照不同时间组处死动物 ,进行股骨的骨密度值测量、病理切片H E染色和茜素红染色病理及OPG/OCIF组织化学染色 ,并进行图像处理和统计分析。结果 在激素性骨坏死早期 ,给药后 2周股骨的OPG/OCIF表达明显降低 (P <0 0 1) ,同时局部骨髓内出现了大量的破骨细胞 ;继而出现明显的骨质丢失 (从 0 375± 0 0 37、0 2 97± 0 0 2 2降至 0 173±0 0 2 4、0 183± 0 0 6 1,P <0 .0 5 )。 结论 糖皮质激素可抑制骨骼局部OPG/OCIF的表达 ,从而促进了破骨细胞分化与活性 ,加剧激素性坏死局部的骨质丢失。

Objective To probe the relationship between the osteoprotegerin/osteoclastogenesis inhibitory factor(OPG/OCIF) expression of bone and the relative bone loss. Methods Steroid induced osteonecrosis model was reproduced in thirty one new Zealand hares, and 24 ones were alive 3 days after administration of endotoxin and methylprednisolone. The alive creatures were divided into control group and trial groups randomly. After the creatures being executed, bone density measurement of the femurs, HE staining, Ca ++ staining and OPG/OCIF immunohistochemistry staining were performed, followed by image processing and statistical analysis. Results In the early period of steroid induced osteonecrosis, the local OPG/OCIF expression significantly decreased, with a large number of osteoclasts in the local medulla, followed by a progressively bone loss. Conclusion Corticosteroid could inhibit OPG/OCIF expression in the bone, increase the activity and differentiation of osteoclast relatively, and worsen bone loss in steroid induced osteonecrosis.