金属铜对骨折愈合作用的实验研究

The effect of metal copper on fracture healing in chicken

目的 观察铜在骨折愈合中的作用。 方法 16 0只出生 70d的鸡饲喂于 32只鸡笼中 ,手术造成鸡翅桡骨 1～ 2mm的缺损 ,然后分为 4组 ,每组 4 0只。每天早上第 1组 (对照组 )鸡胃灌0 8%羧甲基纤维素钠溶液 (Na CMC) ,其余 3组分别胃灌不同剂量的金属铜粉、0 8%羧甲基纤维素钠悬浮液 ,低中高剂量组铜的剂量分别为 2 0、4 0、80mg/kg体重。饲料中含铜 5 4mg/kg饲料 ,所有鸡均自由进食和饮水。分别于手术后的第 14、2 1、2 8和 35d每组各处死 10只鸡。收集肝脏测定其中铜和锌的含量 ;CT桡骨 ,并对骨痂中的骨灰度进行定量测定 ;桡骨的生物力学性能由三点弯曲实验所得的结果进行分析 ;测定骨痂中的钙、镁、磷、铜、锌和铁的含量。 结果 骨痂的灰度值随铜的剂量和饲喂时间的增加而增加 ,术后 2 1d和 35d ,高铜剂量组的骨痂灰度值显著高于对照组 ,分别为 (90 9±2 2 0 )比 (5 97± 15 5 ) ;和 (973± 10 0 )HU比 (76 3± 179)HU(P <0 0 5 )。术后 35d ,中剂量和高剂量组骨痂中钙和镁的含量均显著高于对照组 ,分别为钙 :(177± 2 6 7)、(176± 2 0 5 )、(137± 34 7)mg/g ;镁 :(2 98± 0 5 7)、(3 0 6± 0 4 6 )、(2 4 3± 0 5 3)mg/g。术后 35d ,高剂量组骨痂中羟脯氨酸的含量显著高于对照组 ,分?

Objective To observe the effect of copper on bone fracture healing. Methods 160 growing chickens aged 70 days were fed in 32 cages, each consisting of five. The fracture with 1～2 mm defects at the bilateral radius of wings was created by osteotomy. The chickens were divided into four groups, 40 each. Every morning, chickens of the first group, served as controls, were fed orally 0 8% Na CMC solution (sodium carboxymethyl cellulose), those of the other three groups were fed with copper Na CMC suspension (copper powder suspended in 0 8% Na CMC) with different doses of 20, 40 and 80 mg Cu/kg body weight. Each group was sacrificed 14,21,28 and 35 days postoperatively. Liver was taken for analysis of Cu and Zn. Both radius were removed by dissection. CT was performed quantitatively for the ment of the gray values of the callus. The biomechanical properties of the healing radius were analyzed by a three point bending test. Afterwards, the contents of calcium, magnesium, phosphorus, copper, zinc, iron and hydroxyproline in the callus were determined. Results The gray values of the callus increased along with the increase of copper dose and the duration of observation. 21 and 35 days after operation, the gray values of the callus were significantly higher in the high Cu group than in the controls respectively (909±220 vs. 597±155; 973±100 vs. 763±179 HU, P <0 05). The level of calcium and magnesium in the callus 35 days after operation was much higher in the mid and high Cu groups than in the controls respectively (Ca:177±26 7, 176±20 5 and 137±34 7 mg/g; Mg: 2 98±0 57,3 06±0 46 and 2 43±0 53 mg/g P <0.05). The contents of hydroxyproline in the callus 35 days after operation were significantly higher in the high Cu group than in the controls (34 23±1 96 vs 32 17±1 93 mg/g respectively). The biomechanical properties of the repaired radius had a better improvement tendency in the three Cu fed groups than in the controls. Conclusion Copper is helpful in fracture healing; however, the effect of copper on fracture healing needs further study.